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Sonia Cecilia Labrador, Salvatore Di Lauro, Soraya Tabera-Bartolome, Girish K Srivastava, Jose-Carlos Pastor, Ivan Fernandez-Bueno; Intravitreal mesenchymal stem cells are safe and well-tolerated in pigmented-rabbit eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1829.
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To evaluate safety and tolerance of intravitreally-injected mesenchymal stem cells in pigmented-rabbit eyes, by ophthalmic follow-up and histological study
Mesenchymal stem cells used in this study (MSV; Citospin SL, Valladolid, Spain) are approved for clinical used by the Spanish Drug Agency (PEI n010-134). Twelve rabbits (Rex Chinchilla-NZW) were randomized into three groups of 4 animals. Groups 1 and 2 received an intravitreal injection into the right eye of 1.5x106 and 0.75x106 MSV, respectively, in 0.05 mL vehicle (Ringer-Lactato-based). In Group 3 (control) only the vehicle was injected. Evaluation of anterior pole by slit-lamp, posterior pole by inverted ophthalmoscopy and intraocular pressure by Tonopen® were performed. Clinical signs were evaluated before MSV injection and weekly during 6 weeks. Clinical findings were graded and scored by the Hackett&McDonald method. Finally, animals were sacrificed and the eyes, liver, spleen and gonads processed for hematoxylin and eosin (HE) and periodic acid of Shiff (PAS) staining and histological evaluation
Clinical data did not show statistical significance between control and MSV-injected rabbit eyes at any time point evaluated (p<0.05). No relevant histological findings related to ocular, liver, spleen and/or gonads modifications were observed in any group. MSV were detected intraocularly in only one animal of the Group 1 at 6 weeks
Mesenchymal stem cells (MSV) are safe and well tolerated after intravitreal injection in pigmented-rabbit eyes. Further studies are necessary to evaluate persistence, distribution and potential extraocular migration
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