June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
γδ T cells regulate the expression of cytokines but not the manifestation of fungal keratitis
Author Affiliations & Notes
  • Liya Wang
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Siyu He
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Hongmin Zhang
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Susu Liu
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Yanting Xie
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Guoming Chen
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Hui Liu
    Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Footnotes
    Commercial Relationships Liya Wang, None; Siyu He, None; Hongmin Zhang, None; Susu Liu, None; Yanting Xie, None; Guoming Chen, None; Hui Liu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1885. doi:
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      Liya Wang, Siyu He, Hongmin Zhang, Susu Liu, Yanting Xie, Guoming Chen, Hui Liu; γδ T cells regulate the expression of cytokines but not the manifestation of fungal keratitis. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1885.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: As an important immunoregulatory cell type, the role of γδ T cells in fungal<br /> keratitis(FK) is unclear. We observed the distribution of γδ T cells in infected corneas in vivo by twophoton microscopy.

Methods: The γδ T cells were depleted by neutralizing antibodies. The cytokine expression profile was obtained by protein arrays to determine the cytokines regulated by γδ T cells. ICAM-1, MIP-2 and IL-17A were evaluated by ELISA assays to confirm the role of γδ T cells in FK. We counted the number of neutrophils, evaluated the volume of fungal hypha and analyzed the manifestation of the disease.

Results: The γδ T cells increased significantly at 36 h and 72 h post fungal infection (P< 0.05) and migrated from the limbus to the infection site. The neutralizing antibodies completely depleted the γδ T cells in 24 h. The depletion of γδ T cells led to upregulation of 25 cytokines and downregulation of 3 cytokines. ICAM-1, MIP-2 and IL-17A changed significantly because of the depletion of γδ T cells (P< 0.05). However, the number of neutrophils, volume of fungal hypha and manifestation of the disease was not affected by the depletion of γδ T cells.

Conclusions: Our results demonstrated that γδ T cells have a role in FK via regulation of some cytokines but did not affect the manifestation of this disease, suggesting that γδ T cells are not the key regulator cells in this disease

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