June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Necrostatin-1eyedrop inhibits sodium iodate induced retinal degeneration in rabbit
Author Affiliations & Notes
  • Choun-Ki Joo
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul
    Seoul St. Mary’s hospital, Seoul, Korea (the Republic of)
  • Jun-Sub Choi
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul
  • Footnotes
    Commercial Relationships Choun-Ki Joo, None; Jun-Sub Choi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 2292. doi:
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    • Get Citation

      Choun-Ki Joo, Jun-Sub Choi; Necrostatin-1eyedrop inhibits sodium iodate induced retinal degeneration in rabbit. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2292.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The aim of this study was to investigate the effect of necrostain-1 on cell death of retinal pigment epithelium and photoreceptor in rabbits

 
Methods
 

In this study, we used pigmented rabbit (3kg, Male, Chinchilla). The retinal degeneration was induced sodium iodate (60mg/kg, IV). Nectostatin-1 (7 Cl-O-Nec1) was administered topically, 2times per day for 1week. After 1 week, electroretinograms (ERGs) were performed for functional test. And then, retinal funduscopies were performed for geographic atrophy. And the rabbit eyes were enucleated for histological examination. To observe cell death, TUNEL assay and electron microscopy were performed

 
Results
 

Sodium iodate induced cell death in rabbit photoreceptor and retinal pigment epithelium. The loss of A-wave and geographic atrophy were observed in sodium iodate injected rabbit eye. The Nec1 eye drop was applied 1 day before sodium iodate injection. In nec-1 pretreated group, the A-wave was remained compare with non-treated group. The cell death and geographic atrophy was inhibited by NEC-1 pretreatment

 
Conclusions
 

In this study, we confirmed rabbit model as a suitable animal model for the functional study. And the necroptosis inhibitor NEC-1 shows inhibition retinal degeneration by sodium iodate. And also, we confirmed retinal function in Nec-1 treated retina. Thus, these finding suggest that the Nec-1 is a therapeutic drug for retinal degeneration

 
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