June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
High-effect induction of human iPS cells into retinal pigment epithelial cells with small molecules
Author Affiliations & Notes
  • Qingjian Ou
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Caixia Jin
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Haibin Tian
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Furong Gao
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Weiye Li
    Department of Ophthalmology, Drexel University Collegeof Medicine, Philadelphia, PA
  • Lixia Lu
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Guo-Tong Xu
    Laboratory of Clinical Visual Sciences and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships Qingjian Ou, None; Caixia Jin, None; Haibin Tian, None; Furong Gao, None; Weiye Li, None; Lixia Lu, None; Guo-Tong Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 2355. doi:
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    • Get Citation

      Qingjian Ou, Caixia Jin, Haibin Tian, Furong Gao, Weiye Li, Lixia Lu, Guo-Tong Xu; High-effect induction of human iPS cells into retinal pigment epithelial cells with small molecules. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2355.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: This study is aimed to establish a more effective method to induce the differentiation of iPS cells into retinal pigment epithelial (RPE) cells with small molecules in serum free medium, in order to facilitate the clinical application of iPS cells derived donor cells.

Methods: Human iPS (hiPS) cells were cultured on the matrigel-coated dish in serum free conditioned medium. The iPS cells were first treated with two small molecules, SB431542 and DMH1, for 7days (dual-Smad) and then with other groups of small molecules at the appropriate time. The medium was changed every two days and the cells were separated with accutase before 18 days once the cells were confluence. RPE-like cells derived from hiPS cells (hiPS-RPE) were collected after 24 days induction. The hiPS-RPE cells were confirmed by Q-PCR, immunohistochemistry and western blot for the expression of RPE related genes and proteins.

Results: Combination of different small molecules can induce hiPS cells differentiate into RPE cells after 24 days, and more than 90 percent induced cells differentiate into RPE cells. The hiPS-RPE cells have the typical polygonal morphology and pigments. Meanwhile, they express the RPE cells specific markers.

Conclusions: We can induce hiPS Cells into RPE-like cells under chemically defined conditions with the combination of small molecules in about 3weeks.

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