June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
The effect of dietary supplementation with omega-3 and omega-6 long-chain polyunsaturated fatty acids on retinal detachment
Author Affiliations & Notes
  • Yoko Okunuki
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Eiichi Hasegawa
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Ryoji Yanai
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Kaylee Smith
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Alexandra Castillejos
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Kip M Connor
    Angiogenesis laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Footnotes
    Commercial Relationships Yoko Okunuki, None; Eiichi Hasegawa, None; Ryoji Yanai, None; Kaylee Smith, None; Alexandra Castillejos, None; Kip Connor, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 25. doi:
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      Yoko Okunuki, Eiichi Hasegawa, Ryoji Yanai, Kaylee Smith, Alexandra Castillejos, Kip M Connor; The effect of dietary supplementation with omega-3 and omega-6 long-chain polyunsaturated fatty acids on retinal detachment. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):25.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Retinal detachment (RD) is a sight threatening disorder characterized by the physical separation of the photoreceptors from the retinal pigment epithelium. As a result, the detached photoreceptors undergo apoptosis and programmed cell death. It has been suggested that omega-3 long-chain polyunsaturated fatty acid (LCPUFA) and its derivatives have the neuroprotective and anti-inflammatory properties. On the other hand, omega-6 LCPUFA has been suggested as pro-inflammatory. In this study, we examined the effect of dietary supplementation, with omega-3 and omega-6 LCPUFAs, on photoreceptor cell death after RD.

Methods: C57BL/6J mice were fed a diet enriched with either omega-3 or omega-6 LCPUFAs for 2 weeks. Then RD was induced by subretinal injection of 4 ul of sodium hyaluronate into C57BL/6J mice. RD-induced photoreceptor cell death was assessed by TUNEL staining at 24, 48, 72hrs, and 7 days after the surgery. Additionally, outer nuclear layer thickness was evaluated 7days after the surgery.

Results: The number of TUNEL positive cells in the outer nuclear layer was not significant in mice fed either omega-3 or omega-6 LCPUFAs 24hrs post retinal detachment (p=0.1). However at 48hrs post detachment omega-3 fed mice had a significant, albeit small, decrease in the number of TUNEL positive cells (p=0.04) compared to their omega-6 fed counterparts. There was no difference in the number of TUNEL positive cells at 72hrs and 7days after RD. Outer nuclear layer thickness at 7 days after RD was not different between omega-3 and omega-6 LCPUFAs fed mice.

Conclusions: We demonstrated that dietary supplementation with omega-3 and omega-6 LCPUFAs does not likely play a significant effect on photoreceptor cell death after RD. This suggests that omega-3 LCPUFA lack a neuroprotective effect on photoreceptor cell death after RD.

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