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Manal M Gabriel, Cindy McAnally, Rhonda Walters, Linda Clark, Monica Crary, John Bartell, Bradley Catalone; Biocidal Efficacy of a New Hydrogen Peroxide Disinfecting Solution Against Clinical Bacterial and Yeast Isolates, and Acanthamoeba Species. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):272.
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© ARVO (1962-2015); The Authors (2016-present)
Lens care products are currently evaluated for efficacy against a panel of microorganisms, which includes five laboratory maintained strains representing keratitis-causing species. This study evaluated the efficacy of a new Hydrogen Peroxide lens care solution with clinical isolates derived from corneal ulcers and microbial keratitis, including strains of Acanthamoeba spp.
The antimicrobial efficacy of a new 3% Hydrogen Peroxide lens care solution was evaluated by the Stand-Alone test method (FDA 510[k] guidance/EN ISO 14729 standard). Clinical bacteria and yeast isolates were obtained from patients with microbial keratitis or corneal ulcers including cytotoxic and invasive strains of Pseudomonas spp., emerging Gram-negative organisms Stenotrophomonas spp. and Achromobacter, clinical ocular species of Staphylococcus (MRSA), Enterobacter, Candida and Acanthamoeba spp. Three lots of the test solution were inoculated to contain a final concentration of 1.0 x 105 - 1.0 x 106 CFU/mL for bacteria and yeast. For Acanthamoeba spp., the test solution was inoculated with 1.0 x 104 - 1.0 x 105 cells/ml. The inoculated solutions were evaluated for viable counts at 6 hours disinfection time. Test controls (no product) provided a baseline organism count by which log reduction values were calculated.
Clinically relevant Pseudomonas, Stenotrophomonas, Achromobacter, Staphylococcus and Enterobacter had an average log reduction of >4.0 logs (0.0-0.3 ±SD) following 6 hour exposure (disinfection time) to the new 3% Hydrogen Peroxide lens care solution. Candida had an average log reduction of 2.7 logs (0.1 ±SD) and Acanthamoeba spp. had an average log reduction of >1.0 logs (0.2 ±SD) at 6 hours. The new Hydrogen Peroxide solution, challenged with ocular clinical isolates, meets the primary criteria of the EN ISO 14729 Stand-alone test, requiring >3 log reduction for bacteria and >1 log reduction for yeast. There are no criteria currently for activity of contact lens care solutions against Acanthamoeba spp.
These results demonstrate that the new 3% Hydrogen Peroxide solution was effective at eliminating or reducing populations of relevant clinical ocular isolates including Acanthamoeba spp.
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