June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Early optic nerve head basal blood flow alterations are different in non-human primate models of optic nerve transection and experimental glaucoma.
Author Affiliations & Notes
  • Grant Cull
    Devers Eye Institute, Portland, OR
  • Simon Thompson
    Devers Eye Institute, Portland, OR
  • Claude F Burgoyne
    Devers Eye Institute, Portland, OR
  • Reinhard Told
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria
  • Lin Wang
    Devers Eye Institute, Portland, OR
  • Footnotes
    Commercial Relationships Grant Cull, None; Simon Thompson, None; Claude Burgoyne, Heidelberg Engineering (F), Heidelberg Engineering (R); Reinhard Told, None; Lin Wang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 2744. doi:
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      Grant Cull, Simon Thompson, Claude F Burgoyne, Reinhard Told, Lin Wang; Early optic nerve head basal blood flow alterations are different in non-human primate models of optic nerve transection and experimental glaucoma.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2744.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To compare the effect of experimental glaucoma (EG) and optic nerve transection (ONT) on optic nerve head (ONH) basal blood flow (BF) differentiating BF changes induced by chronic elevated IOP from non-IOP associated optic neuronal degeneration.

 
Methods
 

In adult NHPs, unilateral ONT (n=5) and chronic IOP elevation (n=15) were induced surgically and by trabecular meshwork laser, respectively.. The contralateral eye served as control (CTL). Baseline (BL) ONH BF measurements at IOP 10 mmHg (excluding large vessels) was measured by Laser Speckle Flowgraphy (n=3-5 times). RNFLT was measured by SDOCT at BL and bi-weekly post-laser or ONT. ONH BF measurements for both eyes were split in groups based on RNFLT loss (percentage vs. BL): 1) BL, 2) <10%, 3) 10% to 20%, 4) 20% to 30%, 5) 30% to 40%, and 6) > 40%. A repeated measure ANOVA and post hoc Fisher’s LSD test assessed ONH BF change (EG vs CTL, and ONT vs CTL).

 
Results
 

ONT eyes developed a linear decrease in BF that correlated strongly with RNFLT loss (R2 = 0.9), and achieved significance once RNFLT loss was >10% (Fisher’s LSD).EG eye bf increased significantly compared to BL (p=0.03 *) while RNFLT loss was <10%.As RNFLT decreased further (10-30%), BF returned to being statistically indistinguishable from BL in the EG eyes and was significant different only after RNFLT loss was > 30%. There was no significant BF change in the CTL eyes at any post treatment time point (Fig.1). There was a significant interaction between stage of RNFLT and treatment in both EG (p<0.0001) and ONT (p=0.001) groups.

 
Conclusions
 

Both ONT and EG models cause RNFLT loss. However, EG chronic IOP elevation produces an early increase in BF through 10% RNFLT loss that is not present in ONT. This indicates the mechanisms underlying early ONH BF change in EG may be different than ONT. It suggests an early autoregulation dysfunction in EG and may reflect primary insults that precede and/or directly contribute to early RGC axon loss. The eventual decrease in ONH BF in EG could be contributed by neural degeneration, as evidenced in the ONT model. The mechanisms of early BF autoregulation in EG are understudy.  

 
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