June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Lipid deposition and oxidized-low density lipoprotein in pterygium
Author Affiliations & Notes
  • Dulei Zou
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Liying Zhang
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Changkai Jia
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Tingting Liu
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
  • Xumin Shang
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Fan Yang
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Zuguo Liu
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Wei Li
    Eye Institute of Xiamen University,Fujian Provincial Key Laboratory of Ophthalmology & Visual Science, Xiamen, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, China
  • Footnotes
    Commercial Relationships Dulei Zou, None; Liying Zhang, None; Changkai Jia, None; Tingting Liu, None; Xumin Shang, None; Fan Yang, None; Zuguo Liu, None; Wei Li, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3061. doi:
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    • Get Citation

      Dulei Zou, Liying Zhang, Changkai Jia, Tingting Liu, Xumin Shang, Fan Yang, Zuguo Liu, Wei Li; Lipid deposition and oxidized-low density lipoprotein in pterygium. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3061.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Previous study has shown abnormal cholesterol metabolism and lipid peroxidation in pterygium, this study was to investigate whether lipid deposition and apolipoprotein peroxidation exist in pterygium.

Methods: Primary pterygium specimens were cut into two parts along the central long axis for frozen and paraffin embedding, respectively. Semi-quantitative reverse transcription-polymerase chain reaction ( RT-PCR ) and western blot ( WB ) were used to detect low density lipoprotein receptor ( LDL-R ), Lectin-like oxidized low density lipoprotein receptor-1 ( LOX-I ) and scavenger receptor that binds phosphatidylsedne and oxidized lipoprotein ( SR-PSOX ). Lipid staining( sudan black B and oil red O ), specific lipid extraction and filipin Ⅲ staining were used to investigate lipid deposition. Immunofluorescence (IF) or Immunohistochemistry ( IHC ) was used to apolipoprotein ( including APOA1, B and E ), malonaldehyde-low density lipoprotein ( MDA-LDL ), oxidized-low density lipoprotein ( OX-LDL ), LDL-R, LOX-1 and SR-PSOX.

Results: We found that lipid staining, filipin Ⅲ staining, APOA1 and APOE were positive in the head area of pteygium. MDA-LDL was positive in the whole pterygium tissue. OX-LDL was weak positive in the superficial parts of pterygium. LOX-1 was expressed in small amount of the samples. LDL-R and PS-OX was not expressed in pterygium.

Conclusions: Lipid, especially esterified cholesterol, APOA1, and APOE were deposited at head area of pterygium. Two different oxidized levels of LDL expressed in pterygium in different level. Lipid deposition and apolipoprotein peroxidation may be engaged in pterygium pathological progress.

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