June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Effects of Quercetin in a Mouse Model of Experimental Dry Eye
Author Affiliations & Notes
  • JaeWook Yang
    Dept of Ophthalmology, InJe University Busan Paik Hosp, Busan
  • Ha Na Oh
    Dept of Ophthalmology, InJe University Busan Paik Hosp, Busan
  • Jihyun Lee
    University, Ocular Neovascular Disease Research Center, Busan, Korea (the Republic of)
  • Chae Eun Kim
    University, Ocular Neovascular Disease Research Center, Busan, Korea (the Republic of)
  • Footnotes
    Commercial Relationships JaeWook Yang, None; Ha Na Oh, None; Jihyun Lee, None; Chae Eun Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3063. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      JaeWook Yang, Ha Na Oh, Jihyun Lee, Chae Eun Kim; Effects of Quercetin in a Mouse Model of Experimental Dry Eye. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3063.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the treatment effect of quercetin on dry eye in a mouse model.

Methods: 0.5% quercetin eye drops were prepared by diluting 10% quercetin with phosphate buffered saline (PBS). Experimental dry eye model was induced in NOD.B10.H2b mice by dessicating stress. The mice were divided into three groups according to the treatment regimen : DS 10D (only desiccation stress for 10 days) ; PBS group; and Quercetin group. Tear volume and corneal irregularity scores were measured at 3, 5, 7 and 10 days after treatment. Haematoxylin and eosin staining(H&E), periodic acid-Schiff staining(PAS) and immunohistochemistry(IHC) were performed at the end of the experiment.

Results: Quercetin group showed significantly increased tear volume and decreased corneal irregularity score compared with PBS group (p<0.05). Quercetin group kept smoothe corneal surface without corneal epithelial detachment in H&E and indicated increased goblet cell density in PAS. Quercetin group also showed significant declines of TNF-α, MMP-2, MMP-9, ICAM-1 and VCAM-1 levels in IHC of the lacrimal gland compared with PBS group.

Conclusions: Quercetin is thought to be helpful in improving the ocular surface disorders of dry eye not only by restoring the corneal epithelial detachment but also by increasing tear volume and goblet cell density. Furthermore, quercetin is effective in the treatment of dry eye related to inflammation.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×