June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Evaluation of the Safety of Eye Wash with EyebonR Washing Liquid
Author Affiliations & Notes
  • Hiroshi Fujishima
    Ophthalmology, Tsurumi Univ School of Dental Med, Yokohama, Japan
  • Masaki Iwashita
    Ophthalmology, Tsurumi Univ School of Dental Med, Yokohama, Japan
  • Hiroko Yano
    R&D Health Division, Kobayashi Pharmaceutical Co., Ltd, Ibaraki, Japan
  • Miho Tanaka
    R&D Health Division, Kobayashi Pharmaceutical Co., Ltd, Ibaraki, Japan
  • Yasuko Santo
    R&D Health Division, Kobayashi Pharmaceutical Co., Ltd, Ibaraki, Japan
  • Murat Dogru
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • Footnotes
    Commercial Relationships Hiroshi Fujishima, Kobayashi Pharmaceutical Co., Ltd. (F); Masaki Iwashita, None; Hiroko Yano, Kobayashi Pharmaceutical Co, Ltd (E); Miho Tanaka, Kobayashi Pharmaceutical Co., Ltd (E); Yasuko Santo, Kobayashi Pharmaceutical Co., Ltd (E); Murat Dogru, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3065. doi:
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      Hiroshi Fujishima, Masaki Iwashita, Hiroko Yano, Miho Tanaka, Yasuko Santo, Murat Dogru; Evaluation of the Safety of Eye Wash with EyebonR Washing Liquid. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3065.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: In Japan, eyewash is a popular remedy for symptoms associated with contact lens wear and for the treatment of cedar pollen related allergic conjunctivitis. EyebonR (Kobayashi Pharmaceutical Co, Osaka, Japan) is a non-preserved anti-inflammatory and anti-histaminic washing liquid containing aminocapron, tocopherol acid ester, vitamin B6, chlorphenylamine, chondroitin acid ester, and dipotassium glycyrrhizate. In this study, we evaluated the safety of EyebonR in rabbits and human volunteers.

Methods: EyebonR eye wash was performed 9 times per a day for 30 seconds in rabbits in one eye with isotonic sodium chloride solution (IS) used as control for the other eye. Fluorescein and lissamine corneal staining scores, and in vivo confocal microscopy examinations of the cornea were performed to evaluate phenotypic and inflammatory changes before and after eye wash. In the human study, non-preserved EyebonR, EyebonR with preservatives, another commercial washing solution (Rohto RizeR, Japan), and IS with preservatives were applied in one eye for 30 seconds 6 times a day. Fluorescein and lissamine corneal staining scores, and fluorophotometry for corneal epithelial permeability were assessed before and after eye wash. Animal studies were conducted in accordance with ARVO guidelines for animal experiments. Human study was IRB reviewed and followed the tenets of the Declaration of Helsinki. Non-paired t tests were used for statistical analyses. A p value less than 5% was considered statistically significant.

Results: In the rabbit study, there was no statistical difference between EyebonR and IS wash in relation to vital staining scores, corneal epithelial cell density and inflammatory cell numbers in in vivo confocal microscopy (p>0.05). In the human study, IS with preservatives showed significantly higher corneal staining scores than other solutions (p<0.05). EyebonR with preservatives also showed a significantly higher staining but was significantly lower than IS with preservatives (p<0.05).

Conclusions: Eye wash liquids with preservatives cause damage on the ocular surface but preservative free liquids show significantly lesser vital staining scores and less perturbation of corneal epithelial permeability•barrier function. EyebonR eye wash appears to be a safe modality with promising future applications in dry eye and allergic eye disease.

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