June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Rebamipide Reduces the Response to Capsaicin in Sensory Ganglion Cells
Author Affiliations & Notes
  • Yoshiaki Tagawa
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Kousuke Noda
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Ryo Miyamoto
    Laboratory of Pharmacology, Graduate School of Veterinary Medicine, Sapporo, Japan
  • Ken-ichi Otsuguro
    Laboratory of Pharmacology, Graduate School of Veterinary Medicine, Sapporo, Japan
  • Erdal Tan Ishizuka
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Saori Inafuku
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Takeshi Ohguchi
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Atsuhiro Kanda
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Susumu Ishida
    Ophthalmology, Hokkaido University, Sapporo, Japan
  • Footnotes
    Commercial Relationships Yoshiaki Tagawa, None; Kousuke Noda, None; Ryo Miyamoto, None; Ken-ichi Otsuguro, None; Erdal Tan Ishizuka, None; Saori Inafuku, None; Takeshi Ohguchi, None; Atsuhiro Kanda, None; Susumu Ishida, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 309. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yoshiaki Tagawa, Kousuke Noda, Ryo Miyamoto, Ken-ichi Otsuguro, Erdal Tan Ishizuka, Saori Inafuku, Takeshi Ohguchi, Atsuhiro Kanda, Susumu Ishida, ; Rebamipide Reduces the Response to Capsaicin in Sensory Ganglion Cells. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):309.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Rebamipide, an amino acid derivative of 2-(1H)-quinolinone, is known to increase mucin production, which reportedly contribute to alleviation of symptoms in patients with dry eye, in conjunctival goblet cells and corneal epithelial cells. However, the mechanism underlying the pharmacological effect of rebamipide in dry eye remains obscure. We previously reported that topical administration of rebamipide ameliorates hyperalgesia in patients with dry eye, indicating that rebamipide might regulate the response of corneal sensory nerve. In this study, we examined the effect of rebamipide on sensory nerve using rattus sensory dorsal root ganglion (DRG) cells.

Methods: After euthanasia, DRG cells were collected from the dorsal spinal nerve root of rattus and cultured with Dulbecco's modified eagle medium. After 24 hours of incubation, the cells were transferred to imaging chamber and stimulated with capsaicin perfusion (10-7 mol/l), agonist of transient receptor potential V1 (TRPV1), for 4 times during experiments. After the first capsaicin stimulation, rebamipide solution (10-4 mol/l, rebamipide group) or vehicle solution (0.1% DMSO, control group) was continuously perfused in the chamber until the end of experiment. Activation of TRPV1, receptor for pain sensation, was evaluated by measurement of intracellular calcium concentration with fura-2 imaging system after capsaicin stimulation. The value of calcium concentration was normalized by the peak concentration of the first administration of capsaicin.

Results: In control group, intracellular calcium concentration originated from the response to capsaicin via TRPV1 in DRG cells, was maintained from the second to the fourth stimulation of capsaicin (0.95 ± 0.07, 1.04 ± 0.08 and 1.05 ± 0.09, arbitrary unit, n = 67). By contrast, in rebamipide group, the concentrations of intracellular calcium were gradually reduced and showed significant decrease at the third and forth stimulation of capsaicin compared with those at the first stimulation of capsaicin alone (0.78 ± 0.12, 0.69 ± 0.06* and 0.55 ± 0.08*, arbitrary unit, *P < 0.05, n = 49).

Conclusions: The current data demonstrated that rebamipide suppressed the response of TRPV1 stimulated with capsaicin in DRG cells. This indicates that rebamipide reduces pain sensation in sensory ganglion cells, which may relate to hyperalgesia and subjective symptoms in patients with dry eye.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×