Purchase this article with an account.
Yang Liu, Erich Knop, Nadja Knop, David A Sullivan, Edward O. List, John J. Kopchick, Juan Ding; Regulation of mouse meibomian gland by the growth hormone/insulin-like growth factor-1 axis.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3200.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We hypothesize that the growth hormone (GH) / insulin-like growth factor 1 (IGF-1) axis plays a significant role in the regulation of the meibomian gland. In support of this hypothesis, we have discovered that IGF-1 activates the PI3K/AKT and inhibits forkhead box O1 signaling pathways, stimulates cellular proliferation, increases the expression of sterol regulatory element-binding protein, promotes lipid accumulation, and inhibits the isotretinoin-induced late apoptosis/necrosis in human meibomian gland epithelial cells. To continue to test our hypothesis, we examined the influence of GH on mouse meibomian gland structure.
We utilized several mouse strains, including: [a] bovine (b) GH transgenic mice with excess GH and IGF-1 action; [b] GH antagonist (A) transgenic mice with decreased GH and IGF-1 action; [c] GH receptor (R) knockout (-/-) mice with no GH activity, and corresponding low levels of IGF-1; and [d] wild type (WT) control mice. Adult mice were sacrificed, and eyelids removed and processed for sectioning and H&E staining. Glandular sizes were quantified with ImageJ.
Our results show that the morphology of WT and bGH meibomian glands appear normal. In contrast, there are striking structural changes in the GH-deficient animals. Many of the GHR-/- and GHA meibomian glands featured hyperkeratinized and thickened ducts, acini inserting into duct walls, and poorly differentiated acini. In addition, preliminary analyses suggest that leukocytes may accumulate in the GH-deficient glands. Grossly, meibomian glands of bGH mice were significantly larger, and those of GHA and GHR-/- mice significantly smaller, than glands of WT mice.
Our findings support our hypothesis that the GH/IGF-1 axis plays a significant role in the control of the meibomian gland. In addition, our data show that GH modulates the size and morphology of this tissue.
This PDF is available to Subscribers Only