June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Conventional Multimodal Imaging Versus “En-Face” OCT2 Angiography: deep inside the chorioretinal vascular tissue
Author Affiliations & Notes
  • Marco Lupidi
    S.Maria Della Misericordia Hospital, Eye Clinic, Perugia, Italy
    Centre Ophtalmologique De L'Odeon, Paris, France
  • Gabriel J Coscas
    Centre Ophtalmologique De L'Odeon, Paris, France
  • Florence Coscas
    Centre Ophtalmologique De L'Odeon, Paris, France
  • Footnotes
    Commercial Relationships Marco Lupidi, None; Gabriel Coscas, None; Florence Coscas, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3325. doi:
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      Marco Lupidi, Gabriel J Coscas, Florence Coscas; Conventional Multimodal Imaging Versus “En-Face” OCT2 Angiography: deep inside the chorioretinal vascular tissue. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3325.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To describe and compare the conventional multimodal imaging findings to the images of all the retinal and choroidal vascular layers obtained with the Spectralis “En-Face” OCT2-ANGIOGRAPHY in healthy subjects without dye injection.

 
Methods
 

Prospective case series of 10 eyes of 10 consecutive asymptomatic patients (7 females, mean age 34 ± 15) were evaluated by the use of a conventional multimodal protocol including Fluorescein Angiography (FA) and Enhanced Depth Imaging (EDI) B-Scan Spectral Domain Optical Coherence Tomography (OCT) and by a new multimodal approach based on the “En-Face” visualization of the macular area obtained with a Spectralis OCT2-ANGIOGRAPHY (Heidelberg Engineering, Heidelberg, Germany). A macro-structural and micro-structural analysis (on a 15 x 10° area) was performed to achieve the difference in detecting capability between the two diagnostic protocols. In order to obtain the best En-Face OCT2-ANGIOGRAPHY images a 25 µm thickness C-scan section was shaped on the ILM profile till the end depth of the foveal depression and then modeled to the BM shape from this level to the Haller’s layer.

 
Results
 

The visualization of the superficial retinal vascular layers was appreciable with both protocols, but the En-Face OCT2-ANGIOGRAPHY offered the possibility to study in detail the deep capillary plexus (in its two components) and the connections with the superficial one. Moreover the En-Face and the OCT2-ANGIOGRAPHY allowed us to distinguish different choroidal vascular layers, including Choriocapillaris and often to highlight the connections between these layers.

 
Conclusions
 

Our study shows the capability of the Spectralis OCT2-ANGIOGRAPHY system to analyze in detail the whole retinal and choroidal vascular tissue in order to be a useful reference in case of pathological conditions.  

 
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