June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Potential Role for Matrix Metalloproteinase-9 in Meibomian Gland Changes in Contact Lens Wearers
Author Affiliations & Notes
  • Waleed Alghamdi
    Brien Holden Vision Institute, Sydney, NSW, Australia
    School of Optometry & Vision Science, University of New South Wales, Sydney, NSW, Australia
  • Maria Markoulli
    School of Optometry & Vision Science, University of New South Wales, Sydney, NSW, Australia
  • Brien A Holden
    Brien Holden Vision Institute, Sydney, NSW, Australia
    School of Optometry & Vision Science, University of New South Wales, Sydney, NSW, Australia
  • Eric B Papas
    Brien Holden Vision Institute, Sydney, NSW, Australia
    School of Optometry & Vision Science, University of New South Wales, Sydney, NSW, Australia
  • Footnotes
    Commercial Relationships Waleed Alghamdi, None; Maria Markoulli, None; Brien Holden, None; Eric Papas, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 333. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Waleed Alghamdi, Maria Markoulli, Brien A Holden, Eric B Papas; Potential Role for Matrix Metalloproteinase-9 in Meibomian Gland Changes in Contact Lens Wearers. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):333.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: In previous work, altered morphology and poorer function of meibomian glands was found in contact lens wearers (CLWs) and previous CL wearers (PWs) than in non-wearers. The mechanism underlying these changes is unclear but as inflammation is known to accompany dry eye disease and contribute to its propagation, it may present as an aetiological component of eyelid tissue change.<br /> In this study, the aim was to investigate how levels of a marker of inflammation in the tears, matrix metalloproteinase-9 (MMP-9), changed, as a function of contact lens wear.

Methods: Flush tears were collected from: (1) three groups of CLWs who had worn lenses on a daily basis for different durations (Short: 2±1 years, Moderate: 5±1 years and Long experience: 10±2 years); (2) a group of PWs who had ceased wear for at least 6 months; (3) healthy non-wearers as a control group. Total MMP-9 concentration was established using sandwich enzyme-linked immunosorbent assay. A one-way analysis of variance (ANOVA) with Bonferroni post-hoc correction was used for statistical comparison.

Results: Flush tear samples of 100 participants (51 females; mean age ± SD: 25.4 ± 4.1) were analysed. CLWs with short experience had MMP-9 concentration (17.26 ± 15.58 ng/mL) that were significantly higher than NWs (2.84 ± 2.32 ng/mL; p <0.05). MMP-9 concentration for CLWs with moderate (11.08 ± 11.5 ng/mL) and long experience (11.36 ± 16.6 ng/mL) and PWs (9.62 ± 17.23 ng/mL) did not differ from NWs.

Conclusions: MMP-9 increases during the early years of CL wear, paralleling the onset of morphological changes in the meibomian glands. This may be an indication of an inflammatory mechanism in meibomian gland dropout in CLWs.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×