June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Corneal Epithelial Pigment Epithelium Derived Factor is Reduced in a Murine Model of Dry Eye Disease
Author Affiliations & Notes
  • Merle Fernandes
    Schepens Eye Research Institute, Boston, MA
  • Brinda Subbarayal
    Schepens Eye Research Institute, Boston, MA
  • Sunil Chauhan
    Schepens Eye Research Institute, Boston, MA
  • Reza Dana
    Schepens Eye Research Institute, Boston, MA
  • Footnotes
    Commercial Relationships Merle Fernandes, None; Brinda Subbarayal, None; Sunil Chauhan, None; Reza Dana, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 335. doi:
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      Merle Fernandes, Brinda Subbarayal, Sunil Chauhan, Reza Dana; Corneal Epithelial Pigment Epithelium Derived Factor is Reduced in a Murine Model of Dry Eye Disease. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):335.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Pigment epithelium derived factor (PEDF) has neurotrophic, anti-inflammatory and anti-angiogenic functions. Here, we aimed to determine whether corneal PEDF is reduced in dry eye disease (DED).

Methods: Dry eye disease was induced in 6-week-old female C57BL/6 mice for 7 and 14 days (n=5 each) by placing them in a controlled environment chamber. Control mice were kept in a normal environment (n=5). Corneas from normal and DED mice were immunohistochemically stained with a primary anti-PEDF antibody (goat) and a secondary anti-goat FITC antibody, and analyzed using fluorescent microscopy. A single cell suspension of corneal epithelial cells was prepared and analyzed for the expression of CD45 and PEDF using flow cytometry.

Results: Immunohistochemistry showed decreased expression of PEDF in the corneal epithelium of DED mice at day 7 and 14 compared to normal mice. In addition, flow cytometry analysis showed that CD45- epithelial cells from DED mice have almost 50% less median fluorescence intensity for PEDF compared to corneal epithelial cells from normal mice.

Conclusions: Corneal epithelium derived PEDF is reduced in a murine model of DED suggesting its potential immunoregulatory role in the homeostasis of the ocular surface.

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