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Amir Hajrasouliha, Guomin Jiang, Hui Shao, Henry J Kaplan; Absence of lymphatic choroidal drainage in the normal and pathologic murine eye.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3385.
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© ARVO (1962-2015); The Authors (2016-present)
Existence of an intraocular lymphatic drainage system in the normal eye is unresolved. We investigated the existence of a choroidal lymphatic draining system in the normal eye and following laser induced choroidal neovascularization (CNV) and experimental autoimmune uveitis (EAU).
C57BL/6 (B6) mice with green fluorescent protein (GFP) under the promoter of a master prospero homeobox protein 1 (PROX-1), a control gene in lymphatic development, were histologically studied for lymphatic channels. CNV was induced by 4 laser spots (50um diameter at 250mV, 0.5s energy) around the optic disc. EAU was induced by immunization with interphotoreceptor retinoid-binding protein (IRBP) 1-20 peptides emulsified with complete Freund’s adjuvant. Immunostaining with Abs specific for lymphatic markers VEGFR-3, podoplanin, LYVE-1 and PROX-1 was performed on choroidal-retinal flat mounts and tissue sections.
: GFP+ cells were not detected in choroidal flat mounts of PROX-1 GFP transgenic mice. 3 out of 5 flat mounts (60%) of the eyes showed LYVE-1 + prepapillary channels that did not extend into the retina. The outer nuclear layer showed PROX-1+, LYVE-1 -, podoplanin - cells without an identifiable vascular channel in flat mounts. LYVE-1 +, podoplanin -, prox-1 - cells were detected 3 and 7 days after laser induced CNV. All lymphatic markers were absent in the choroid and retina2 and 4 weeks after EAU induction.
No choroidal lymphatic drainage was identifiable in the normal murine eye or in pathologic conditions. The prepapillary lymphatic channels may be an extension of dural lympathics into the eye.
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