June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Absence of lymphatic choroidal drainage in the normal and pathologic murine eye.
Author Affiliations & Notes
  • Amir Hajrasouliha
    Ophthalmology, University of Louisville, Louisville, KY
  • Guomin Jiang
    Ophthalmology, University of Louisville, Louisville, KY
  • Hui Shao
    Ophthalmology, University of Louisville, Louisville, KY
  • Henry J Kaplan
    Ophthalmology, University of Louisville, Louisville, KY
  • Footnotes
    Commercial Relationships Amir Hajrasouliha, None; Guomin Jiang, None; Hui Shao, None; Henry Kaplan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3385. doi:
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      Amir Hajrasouliha, Guomin Jiang, Hui Shao, Henry J Kaplan; Absence of lymphatic choroidal drainage in the normal and pathologic murine eye.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3385.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Existence of an intraocular lymphatic drainage system in the normal eye is unresolved. We investigated the existence of a choroidal lymphatic draining system in the normal eye and following laser induced choroidal neovascularization (CNV) and experimental autoimmune uveitis (EAU).

Methods: C57BL/6 (B6) mice with green fluorescent protein (GFP) under the promoter of a master prospero homeobox protein 1 (PROX-1), a control gene in lymphatic development, were histologically studied for lymphatic channels. CNV was induced by 4 laser spots (50um diameter at 250mV, 0.5s energy) around the optic disc. EAU was induced by immunization with interphotoreceptor retinoid-binding protein (IRBP) 1-20 peptides emulsified with complete Freund’s adjuvant. Immunostaining with Abs specific for lymphatic markers VEGFR-3, podoplanin, LYVE-1 and PROX-1 was performed on choroidal-retinal flat mounts and tissue sections.

Results: : GFP+ cells were not detected in choroidal flat mounts of PROX-1 GFP transgenic mice. 3 out of 5 flat mounts (60%) of the eyes showed LYVE-1 + prepapillary channels that did not extend into the retina. The outer nuclear layer showed PROX-1+, LYVE-1 -, podoplanin - cells without an identifiable vascular channel in flat mounts. LYVE-1 +, podoplanin -, prox-1 - cells were detected 3 and 7 days after laser induced CNV. All lymphatic markers were absent in the choroid and retina2 and 4 weeks after EAU induction.

Conclusions: No choroidal lymphatic drainage was identifiable in the normal murine eye or in pathologic conditions. The prepapillary lymphatic channels may be an extension of dural lympathics into the eye.

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