June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Long-term results of autologous transplantation of cultivated limbal epithelium in limbal stem cell deficiency
Author Affiliations & Notes
  • Simone Scholz
    Department of Ophthalmology, University Hospital Essen, Essen, Germany
  • Khaleda Hestermann
    Department of Ophthalmology, University Hospital Essen, Essen, Germany
  • Henning Thomasen
    Department of Ophthalmology, University Hospital Essen, Essen, Germany
  • Klaus-Peter Steuhl
    Department of Ophthalmology, University Hospital Essen, Essen, Germany
  • Daniel Meller
    Department of Ophthalmology, University Hospital Essen, Essen, Germany
  • Footnotes
    Commercial Relationships Simone Scholz, None; Khaleda Hestermann, None; Henning Thomasen, None; Klaus-Peter Steuhl, None; Daniel Meller, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3479. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Simone Scholz, Khaleda Hestermann, Henning Thomasen, Klaus-Peter Steuhl, Daniel Meller; Long-term results of autologous transplantation of cultivated limbal epithelium in limbal stem cell deficiency. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3479.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To report the long-term clinical outcome of on intact amniotic membrane expanded autologous limbal epithelial cells for ocular surface reconstruction in limbal stem cell deficiency (LSCD). <br /> <br />

Methods: Sixty one eyes of 59 patients (49 male and 12 female) with LSCD were treated by transplantation of autologous limbal epithelial cells expanded on intact AM. The etiology of LSCD was chemical and thermal burns (n=38), pterygium (n=8), mitomycin C induced LSCD and tumor excisison (n=11), severe infectious keratitis (n=3), perforating injury, Epidermolysis bullosa, and superficial keratectomy (each n=1). Only eyes with a follow-up time of at least 4 months were included in the analysis. The main outcome measures were restoration of ocular surface integrity and improvement of visual acuity (VA).<br />

Results: The mean follow-up time was 40,2 ± 31.2 months. An entirely stable corneal surface was reconstructed in 48 (78,7%) eyes. Visual acuity increased significantly in 45 (73,8%) eyes, was stable in 10 (16,4%) eyes and decreased in six eyes (9,8%). The mean visual acuity increased significantly (p<0.0001) from preoperative 1.5±0.89 LogMAR to 0.8±0.65 LogMAR.

Conclusions: Transplantation of on intact AM cultivated limbal epithelium restores a stable corneal surface and results in a significant increase of visual acuity in most cases of LSCD. Autologous transplantation of cultivated limbal epithelium show after long-term follow-up an excellent prognosis and outcome.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×