June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Cytokines and chemokines profile in aqueous humor of PACG eyes
Author Affiliations & Notes
  • Roopam Duvesh
    Department of Genetics, Aravind Medical Research Foundation, Madurai, India
  • Krishnadas SR
    Glaucoma Clinic, Aravind Eye Hospital, Madurai, India
  • George Puthuran
    Glaucoma Clinic, Aravind Eye Hospital, Madurai, India
  • Sharmila Rajendrababu
    Glaucoma Clinic, Aravind Eye Hospital, Madurai, India
  • Rengaraj Venkatesh
    Glaucoma Clinic, Aravind Eye Hospital, Madurai, India
  • Srinivasan Kavitha
    Glaucoma Clinic, Aravind Eye Hospital, Madurai, India
  • Pradeep Y Ramulu
    Glaucoma Division, Johns Hopkins Hospital, Baltimore, MD
  • Robert Wojciechowski
    Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD
    Inherited Disease Research Branch, National Human Genome Research Institute, Baltimore, MD
  • Periasamy Sundaresan
    Department of Genetics, Aravind Medical Research Foundation, Madurai, India
  • Footnotes
    Commercial Relationships Roopam Duvesh, None; Krishnadas SR, None; George Puthuran, None; Sharmila Rajendrababu, None; Rengaraj Venkatesh, None; Srinivasan Kavitha, None; Pradeep Ramulu, None; Robert Wojciechowski, None; Periasamy Sundaresan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3482. doi:
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      Roopam Duvesh, Krishnadas SR, George Puthuran, Sharmila Rajendrababu, Rengaraj Venkatesh, Srinivasan Kavitha, Pradeep Y Ramulu, Robert Wojciechowski, Periasamy Sundaresan, ; Cytokines and chemokines profile in aqueous humor of PACG eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3482.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To determine cytokine and chemokine levels in the aqueous humor of Primary angle closure glaucoma (PACG) and cataract patients.<br /> <br />

 
Methods
 

Aqueous humor samples were collected from 19 subjects with PACG and 19 cataract patients as controls. The levels of 27 cytokines, chemokines and growth factors were measured in the aqueous humor samples of study subjects using multiplex bead immunoassay (Bio-Rad). Data on patient demographics and preoperative intraocular pressure (IOP) were also collected. Differences in cytokine concentration between PACG cases and cataract controls were compared using a Wilcoxon rank sum test and a t test comparing log2-transformed data. A 2-sided Bonferroni-adjusted threshold of p=0.002 was used for statistical significance.<br />

 
Results
 

The aqueous concentrations of 27 cytokines, chemokines and growth factors were simultaneously measured for both PACG group and cataract group. PACG group showed higher levels of Macrophage Inflammatory Proteins (MIP)-1β (Median= 51.29 pg/ml) as compared to cataract controls (Median= 23.24 pg/ml; p=0.0002). Seven cytokines (IL-2, IL-4, IL-15, G-CSF, IFN-γ, PDGF-BB and RANTES) were below the limits of detection in few samples. IL-5,IL-6,IL-7,IL-8,IL-9,IL-10,IL-12,IL-13,IL-17,Eotaxin, basic-FGF,GM-CSF,IP-10,MCP-1,MIP-1α,MIP-1β,TNF-α and VEGF were detected in all the study samples.<br />

 
Conclusions
 

Concentration of the MIP-1β is significantly higher in the aqueous humor of PACG patients when compared to cataract patients indicating MIP-1β might have possible role in angle closure glaucoma.<br />

 
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