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Xiang-Jia Zhu, Ke-Ke Zhang, Wen-Wen He, Jin Yang, Yi Lu; Proinflammatory status in the aqueous humor of high myopic cataract eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3503.
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© ARVO (1962-2015); The Authors (2016-present)
To detect inflammatory cytokines expressed in the aqueous humor (AH) of high myopic cataract (HMC) patients.
In the screening stage, AH samples from 15 age-related cataract (ARC) patients and 15 HMC patients were collected and assayed using a RayBio Human Cytokine Antibody Array. The cytokines detected by the screening assays were verified using a Bio-Plex Suspension Array System in AH samples obtained from 35 ARC patients and 45 HMC patients.
The cytokine antibody array showed that the expression level of interleukin-1 receptor antagonist (IL-1ra) in the AH was higher in ARC than in HMC, whereas opposite trends were found for monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T-cell expressed and presumably secreted (RANTES), IL-8, platelet-derived growth factor-BB, and IL-6 (all P < 0.05). In the verification assay using the suspension cytokine array, only the expression levels of IL-1ra and MCP-1 were significantly different between ARC and HMC groups (P =0.014 and 0.038, respectively).
The expression of IL-1ra was significantly lower and the expression of MCP-1 was significantly higher in the AH of HMC than in ARC, indicating a proinflammatory status in the anterior chamber of HMC eyes.
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