June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Proinflammatory status in the aqueous humor of high myopic cataract eyes
Author Affiliations & Notes
  • Xiang-Jia Zhu
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China
  • Ke-Ke Zhang
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China
  • Wen-Wen He
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China
  • Jin Yang
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China
  • Yi Lu
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China
  • Footnotes
    Commercial Relationships Xiang-Jia Zhu, None; Ke-Ke Zhang, None; Wen-Wen He, None; Jin Yang, None; Yi Lu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3503. doi:
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    • Get Citation

      Xiang-Jia Zhu, Ke-Ke Zhang, Wen-Wen He, Jin Yang, Yi Lu; Proinflammatory status in the aqueous humor of high myopic cataract eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3503.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To detect inflammatory cytokines expressed in the aqueous humor (AH) of high myopic cataract (HMC) patients.

Methods: In the screening stage, AH samples from 15 age-related cataract (ARC) patients and 15 HMC patients were collected and assayed using a RayBio Human Cytokine Antibody Array. The cytokines detected by the screening assays were verified using a Bio-Plex Suspension Array System in AH samples obtained from 35 ARC patients and 45 HMC patients.

Results: The cytokine antibody array showed that the expression level of interleukin-1 receptor antagonist (IL-1ra) in the AH was higher in ARC than in HMC, whereas opposite trends were found for monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T-cell expressed and presumably secreted (RANTES), IL-8, platelet-derived growth factor-BB, and IL-6 (all P < 0.05). In the verification assay using the suspension cytokine array, only the expression levels of IL-1ra and MCP-1 were significantly different between ARC and HMC groups (P =0.014 and 0.038, respectively).

Conclusions: The expression of IL-1ra was significantly lower and the expression of MCP-1 was significantly higher in the AH of HMC than in ARC, indicating a proinflammatory status in the anterior chamber of HMC eyes.

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