June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Investigating the Effect of PAX6 Gene Expression on Differentiation of CD133+Human Cord Blood Stem Cells
Author Affiliations & Notes
  • Sahar Balagholi
    Iranian Blood Transfusion Organization, Tehran, Iran (the Islamic Republic of)
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Sedigheh Amini Kafiabadi
    Iranian Blood Transfusion Organization, Tehran, Iran (the Islamic Republic of)
  • Zahra-Soheila Soheili
    National Institute of Genetic Engineering and Biotechnology, Tehran, Iran (the Islamic Republic of)
  • Mozhgan Rezaeikanavi
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Shahram Samiei
    Iranian Blood Transfusion Organization, Tehran, Iran (the Islamic Republic of)
  • Footnotes
    Commercial Relationships Sahar Balagholi, None; Sedigheh Amini Kafiabadi, None; Zahra-Soheila Soheili, None; Mozhgan Rezaeikanavi, None; Shahram Samiei, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3581. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Sahar Balagholi, Sedigheh Amini Kafiabadi, Zahra-Soheila Soheili, Mozhgan Rezaeikanavi, Shahram Samiei; Investigating the Effect of PAX6 Gene Expression on Differentiation of CD133+Human Cord Blood Stem Cells. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3581.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To assess the influence of PAX6 gene expression on differentiation of CD133+ human cord blood stem cells.

Methods: Human cord blood mononuclear cells were isolated by ficoll. CD133+ cells were obtained using the CD133MicroBead Kit in combination with the autoMACS Separator and then cultured in the Stem Span media. HEK293T packaging cells were co-transfected with PLEX-MCS, PsPAX2, and pMD2G via calcium phosphate method. The resultant lentiviral vectors were collected and concentrated by PEG 6000. The appropriate amount of viruses was used for infecting CD133+ cells. The successful transduced cells were selected by puromycin resistance. After two weeks, the expression of rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins were assayed by immunocytochemistry.

Results: Two weeks after transfection, CD133+ cells expressed rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins, as compared to the HEK293T cells that expressed rhodopsin and nestin.

Conclusions: Transfected CD133+ human cord blood stem cells with PAX6 gene are able to differentiate into progenitor retinal neural- and ganglion-like cells.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×