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Shaomei Wang, Bin Lu, YuChun Tsai, sergey Girman, Lin Shen, Benjamin Bakondi; Non-invasive administration of MSCs preserves vision in rodent model for retinal degeneration. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3602.
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© ARVO (1962-2015); The Authors (2016-present)
Mesenchymal stem cells (MSCs) have been showed effective in preserving vision after systemic administration into the Royal College Surgeon (RCS) rats with primary defect in the retinal pigment epithelium. The major advantage of using MSC is that they can be delivered intravenously, which offers extensive protection compared with local injection and can be redosed without side effect. Here we investigate whether intravenous injection of MSCs into another rodent model with primary defect in the photoreceptors-S334ter rats would have protective effect.
MSC were isolated from S334ter and Long Evan’ rats as described before. S334ter rats at postnatal day (p) 17-19 received intravenous injection of 1million MSCs. The following groups were studied: 1) MSC without pretreatment; 2) MSCs were pretreated before administration; 3) subretinal injection of cells/medium into the S334ter rats at P16, on the next day, intravenous injection of MSC. The efficacy of above treatments were examined by optokinetic response, electroretinogram and luminance threshold recording from the superior colliculus; and retinal histology.
Intravenous injection of MSC without any treatment into S334ter rats can preserve photoreceptors globally and visual function. Photoreceptor layers were 2-3 cells thick covering whole eye, compared with untreated eye, there was a discontinuous layer of photoreceptors remaining. Visual function assessed by OKR, ERG and luminance threshold recording was also significantly better than controls. Study is ongoing to investigate the mechanism of action of MSCs in preserving vision.
Intravenous administration of MSCs offers global protection of photoreceptors in rodent models for retinal degeneration regardless of the genetic mutations.
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