June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Hyperspectral Autofluorescence (AF) Characterization of Drusen in Donor Eyes with Age-Related Macular Degeneration (AMD)
Author Affiliations & Notes
  • Yuehong Tong
    Ophthamology, New York University, Bronx, NY
  • Tal Ben Ami
    Ophthamology, New York University, Bronx, NY
  • Ansh Johri
    Ophthamology, New York University, Bronx, NY
  • Robert Post
    Ophthamology, New York University, Bronx, NY
  • Zsolt Ablonczy
    Ophthalmology, Medical University of South Carolina, Charleston, SC
  • Paul Sajda
    Department of Biomedical Engineering, Columbia University, New York, NY
  • Christine A Curcio
    Ophthalmology, University of Alabama at Birmingham School of Medicine, Birmingham, AL
  • Thomas Ach
    Ophthalmology, University of Alabama at Birmingham School of Medicine, Birmingham, AL
  • Theodore Smith
    Ophthamology, New York University, Bronx, NY
  • Footnotes
    Commercial Relationships Yuehong Tong, None; Tal Ben Ami, None; Ansh Johri, None; Robert Post, None; Zsolt Ablonczy, None; Paul Sajda, None; Christine Curcio, None; Thomas Ach, None; Theodore Smith, Advanced Cell Technologies (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3956. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yuehong Tong, Tal Ben Ami, Ansh Johri, Robert Post, Zsolt Ablonczy, Paul Sajda, Christine A Curcio, Thomas Ach, Theodore Smith; Hyperspectral Autofluorescence (AF) Characterization of Drusen in Donor Eyes with Age-Related Macular Degeneration (AMD). Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3956.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

Drusen are one of the hallmark lesions of AMD. Our purpose is to characterize drusen by their spectral AF signatures, for a better molecular understanding of AMD.

 
Methods
 

Retinal pigment epithelium (RPE)/Bruch’s membrane (BrM)-flatmounts were prepared from 5 donors with AMD. Hyperspectral AF imaging was performed at 11 locations containing drusen at 2 excitation bands, 436-460 and 480-510 nm, with emissions captured between 420 and 700 nm in 10 nm intervals, with the Nuance FX camera. Abundant individual spectra with corresponding spatial localizations were recovered with custom software based on a nonnegative tensor factorization algorithm [1].

 
Results
 

In a typical example the original AF image (RGB) is presented with 5 spatial abundances (Fig. 1) of 5 recovered individual spectra (Fig. 2). In particular, spectrum C4 (Fig. 2, cyan line) localizes precisely and specifically to the drusen and also a diffuse region (upper left Fig. 1, abundance C4) with a peak wavelength of 510 nm. A spectrum with this characteristic peak and shape was recovered from all drusen and variably from diffuse regions in 10 of the 11 locations, with one spectrum peaking at 490 nm. Further, the dominant fluorophore (spectrum C1 and abundance C1 herein) consistently shows moderate co-localization with drusen, indicating this fluorophore overlying as granules and/or within drusen. This fluorophore spectrum is remarkably blue-shifted about 50 nm from the corresponding dominant fluorophore spectrum recovered from previously reported normal donors [1].

 
Conclusions
 

With hyperspectral AF image analysis, we found a single spectral AF signature for drusen and diffuse sub-RPE deposits in AMD that appears to be highly sensitive for drusen. The possibility that this signal also localizes to basal linear deposit is being addressed. A second drusen-associated AF spectral signature shares commonalities with RPE lipofuscin. These hyperspectral AF characterizations of drusen may aid the molecular understanding of AMD.<br /> 1. Smith RT, et. al. Biomed Opt Express. 2014;5(12):4171-85.  

 
Fig. 1. RPE/BrM flatmount from an 84-year-old female donor with AMD. Original RGB and abundance images (labeled C1-C5) corresponding to the individual recovered spectra in Fig 2.
 
Fig. 1. RPE/BrM flatmount from an 84-year-old female donor with AMD. Original RGB and abundance images (labeled C1-C5) corresponding to the individual recovered spectra in Fig 2.
 
 
Fig. 2. Corresponding spectra recovered from 436 nm (solid lines) and 480 nm (dashed lines) excitations.<br />
 
Fig. 2. Corresponding spectra recovered from 436 nm (solid lines) and 480 nm (dashed lines) excitations.<br />

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×