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Alexander Pinhas, Moataz M Razeen, Theodora Danias, Nikhil Menon, Nadim Choudhury, Matthew Field, Joseph Panarelli, Richard B Rosen; Macular Mitochondrial Flavoprotein Autofluorescence in Eyes with Primary Open Angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3983.
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© ARVO (1962-2015); The Authors (2016-present)
Primary open angle glaucoma (POAG) is a progressive optic neuropathy involving mitochondrial dysfunction revealed by characteristic structural changes to the optic nerve head and retinal nerve fiber layer, and functional visual field alterations. This study was designed to quantitatively assess mitochondrial dysfunction in the maculae of patients with POAG compared to healthy controls using flavoprotein autofluorescence (FPF) measured by the Retinal Metabolic Analysis (RMA) (OcuSciences, Ann Arbor, MI).
9 POAG patients (17 eyes; mean age 63, range 56-68 years) and 23 healthy subjects (46 eyes; mean age 60, range 55-69 years) were imaged with RMA. All POAG eyes were currently receiving treatment with intraocular pressure (IOP)-lowering medications. IOP was measured using the Tono-Pen and cup-to-disc ratio (CDR) was estimated using SD-OCT for each eye. Mitochondrial FPF was calculated per eye as a mean of 5 consecutive RMA readings, within a 15-degree circular region centered at the fovea.
FPF for the 9 non-fellow POAG eyes was significantly higher than that of the 23 non-fellow age-matched healthy eyes (564±92 versus 459±76, t(30)=3.31, p=0.0024). Results are reported as group averages ± SD. Greater variability between fellow eye FPF signal asymmetry was also observed in the 8 POAG patients, compared to that in the 23 healthy subjects (18.5±28.7% versus 6.3±5.3%). POAG FPF results showed a weak positive correlation with IOP and CDR.
FPF measured by the RMA appears sensitive to the increased mitochondrial dysfunction in POAG. Ongoing studies exploring the use of RMA for assessing adequacy of therapy in POAG may help define its potential clinical value.
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