Purchase this article with an account.
Antonio Bautista-Hernandez, Beatriz Buentello-Volante, Victor Manuel Bautista, José Luis Gómez Olivares; Effect of the interaction Fusarium solani-Staphylococcus aureus over limbocorneal fibroblasts.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4056.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To study the effect of the interaction Fusarium solani-Staphylococcus aureus over limbocorneal fibroblasts.
The limborcorneal fibroblasts were obtained from limbocorneal tissue and grown in media DMEM-F12 supplemented with serum fetal bovine 10%, were evaluated expression of vimentin and cytokeratin. F. solani and S. aureus were isolated from human corneal ulcers. The microorganisms were identified by classical microbiology. The biofilm formation was evaluated in media DMEM-F12 by the Christensen method and fluorescence microscopy. Fibroblasts were exposed to S. aureus and/or F. solani to evaluate the expression of CD34 and production of INFγ.
According to classical microbiology studies, bacterial strain corresponded to S. aureus and fungal strain to F. solani. Fluorescence microscopy showed a formed biofilm by the interactions between S. aureus and F. solani, that generated a extracellular matrix. The limbocorneal fibroblasts had characteristic morphology, vimentin positive and cytokeratin negative. The expression of CD34 decreased and increased content of INFγ with stimuli of S. aureus, F. solani and S. aureus-F. solani.
Microorganism S. aureus and F. solani showed ability to form biofilm. The limbocorneal fibroblasts were vimentin positive and cytokeratin negative. Expression of CD34 was decreased and increased INFγ production with stimuli of S. aureus, F. solani and S. aureus-F. solani.
This PDF is available to Subscribers Only