June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
The role of Decorin in experimental proliferative vitreoretinopathy model
Author Affiliations & Notes
  • Kazuhiko Umazume
    Ophthalmology & Visual Sciences, Tokyo Medical University, Tokyo, Japan
  • Hiroki Takahashi
    Ophthalmology & Visual Sciences, Tokyo Medical University, Tokyo, Japan
  • Naoyuki Yamakawa
    Ophthalmology & Visual Sciences, Tokyo Medical University, Tokyo, Japan
  • Hiroshi Goto
    Ophthalmology & Visual Sciences, Tokyo Medical University, Tokyo, Japan
  • Footnotes
    Commercial Relationships Kazuhiko Umazume, None; Hiroki Takahashi, None; Naoyuki Yamakawa, None; Hiroshi Goto, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4241. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Kazuhiko Umazume, Hiroki Takahashi, Naoyuki Yamakawa, Hiroshi Goto; The role of Decorin in experimental proliferative vitreoretinopathy model. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4241.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: The cytokine transforming growth factor-ß (TGF-ß) plays a major role to tissue fibrosis. Decorin, a naturally occurring TGF-ß inhibitor, can bind activated TGF-ß and suppress TGF-ß activity. We sought to invesitigate the role of decorin in experimental vitreoretinopathy and to develop novel methods of prevention.

Methods: The effect of decorin on retinal pigment epithelial (RPE) sheet growth was determined by measuring enlargement of cultured RPE sheets in the presence or absence of decorin. Epithelial-mesenchymal transition (EMT) of RPE cells was assessed by expression of S100A4 and alpha smooth actin expression (αSMA). Type I collagen contraction assay were utilized to examine the effect of decorin on extracellular matrix (ECM) contraction.

Results: Decorin did not prevent the growth of RPE sheets and EMT, however, suppressed the expression of αSMA in RPE sheets. Moreover, 150μg/ml of decorin inhibited nearly 70% of collagen gel contraction.

Conclusions: Decorin significantly inhibited PVR-related RPE changes in vitro. Theses data suggested that decorin may be effective in the prevention of PVR.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×