June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Altered Retinal Structure and Function in Toll-like Receptor 4 (TLR4) Knockout Mice
Author Affiliations & Notes
  • Nicolas Cuenca
    Physiology, Genetics and Microbiology, University of Alicante SPAIN, Alicante, Spain
  • Agustina Noailles
    Physiology, Genetics and Microbiology, University of Alicante SPAIN, Alicante, Spain
  • Emilio De Juan
    Physiology, Genetics and Microbiology, University of Alicante SPAIN, Alicante, Spain
  • Victoria Maneu
    Optics, Pharmacology and Anatomy, University of Alicante, Alicante, Spain
  • Violeta Gomez-Vicente
    Optics, Pharmacology and Anatomy, University of Alicante, Alicante, Spain
  • Pedro Lax
    Physiology, Genetics and Microbiology, University of Alicante SPAIN, Alicante, Spain
  • Footnotes
    Commercial Relationships Nicolas Cuenca, None; Agustina Noailles, None; Emilio De Juan, None; Victoria Maneu, None; Violeta Gomez-Vicente, None; Pedro Lax, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 433. doi:
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      Nicolas Cuenca, Agustina Noailles, Emilio De Juan, Victoria Maneu, Violeta Gomez-Vicente, Pedro Lax; Altered Retinal Structure and Function in Toll-like Receptor 4 (TLR4) Knockout Mice. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):433.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Toll-like receptors, key mediators in the inflammatory response, have been recognized as modulators of neuronal development and survival. The objective of this study is to assess the role of TLR4 in the structure and function of the mouse retina.

Methods: Retinal responsiveness and visual acuity were evaluated by electroretinography and optomotor test, respectively, in TLR4-/- knockout and wild type C57BL/6J mice at P20, P30 and P60. Retinal structure was also assessed at P30 in both animal strains by immunohistochemical techniques and confocal microscopy.

Results: TLR4-/- mice showed reduced retinal responsiveness, as compared to wild type animals. The scotopic a- and b-waves were significantly lower in TLR4-defective mice than in wild type animals at all ages tested (P20, P30 and P60) (ANOVA, p<0.05). Visual acuity was also lower in TLR4-/- compared to C57BL/6J mice, with significant differences at P60 (ANOVA, p<0.05). Vertical retinal sections from P30 TLR4-/- mice showed lower density of bipolar cells and less synaptic contacts between photoreceptors and bipolar cells (ANOVA, p <0.05 in both cases) than in the wild type.

Conclusions: Absence of the innate immune receptor TLR4 alters the structure and impairs the function in the mouse retina.

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