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Rong Wen, Xin Xia, Tsung-Han Chou, Yiwen Li, Vittorio Porciatti; Protection of pattern electroretinogram (PERG) by oncostatin M after optic nerve injury in mice. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4364.
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Oncostatin M (OSM) is a member of the IL-6 family of cytokines, which includes interleukin 6 (IL-6), IL-11, ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), cardiotropin 1 (CT-1), and cardiotrophin-like cytokine (CLC). We have shown previously that OSM protects rod and cone photoreceptors. The present work investigates the effect of OSM on preserving PERG after optic nerve (ON) crush in mice.
ON-crush was performed unilaterally on 2 months old BALB/C mice. In the treated groups, eyes were injected with recombinant human OSM (3µg in 2µl) or CNTF (3µg in 2µl) immediately after ON crush. The control eyes were injected with 2µl phosphate buffered saline (PBS). PERG was elicited with visual stimuli of contrast-reversing 15 cm. Photopic flash ERGs were also recorded with undilated pupils in response to strobe flashes of 20 cd/m²/s superimposed on a steady background light of 12 cd/m² and presented within a Ganzfeld bowl. Averaged PERG and FERG were analyzed to evaluate the peak-to-trough amplitudes.
PERG was recorded before ON crush as baseline and at 8, 15, 22 days after ON-crush. Baseline PERG waveforms and amplitudes in BALB/C mice ranged between 15 and 22 µV. PERG amplitudes of individual mice were normalized to the mean baseline amplitude of each group. Eight days after ON-crush in PBS-treated eyes, PERG amplitude decreased sharply to 15% of the baseline level. In both OSM- and CNTF-treated eyes, however, the PERG amplitudes were significantly higher (P=0.003) than that of the PBS-treated eyes. By day 15, the PERG amplitudes of OSM- and CNTF-treated eyes decreased to the levels close to the noise range, not statistically different from PBS-treated eyes. The PERG amplitudes of all groups were in the noise range 22 days after ON-crush and no significant difference was found among the three groups. No significant changes were observed in photopic flash ERGs over time after ON-crush in any experimental groups.
A single injection of OSM or CNTF after ON-crush improves RGC electrophysiological activity. These results provide proof-of-concept for using neurotrophic factors OSM and CNTF for RGC degenerative diseases, including glaucoma and acute optic nerve trauma.
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