June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Spatial Variation in Vitreous Oxygen Consumption
Author Affiliations & Notes
  • Karthik Murali
    University of Southern California, Los Angeles, CA
  • Hossein Nazari
    University of Southern California, Los Angeles, CA
  • Dongyang Kang
    California Institute of Technology, Pasadena, CA
  • Nicholas Scianmarello
    California Institute of Technology, Pasadena, CA
  • Yu-Chong Tai
    California Institute of Technology, Pasadena, CA
  • Mark S Humayun
    University of Southern California, Los Angeles, CA
  • Amir H Kashani
    University of Southern California, Los Angeles, CA
  • Footnotes
    Commercial Relationships Karthik Murali, None; Hossein Nazari, None; Dongyang Kang, None; Nicholas Scianmarello, None; Yu-Chong Tai, None; Mark Humayun, None; Amir Kashani, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4416. doi:
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      Karthik Murali, Hossein Nazari, Dongyang Kang, Nicholas Scianmarello, Yu-Chong Tai, Mark S Humayun, Amir H Kashani; Spatial Variation in Vitreous Oxygen Consumption. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4416.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The vitreous consumes oxygen via an ascorbic acid reaction with molecular oxygen. The spatiotemporal characteristics of this reaction, specifically within the eye, are not fully understood. We investigated the spatiotemporal variation of vitreous oxygen consumption in porcine cadaver eyes.

 
Methods
 

Whole porcine eyes were obtained from a local slaughterhouse within 48 hours of enucleation. The native cornea and the lens were removed and replaced with a keratoprosthesis. Using a standard sclerotomy, a custom-made, semi-permeable oxygen delivery source was placed in two separate intravitreal locations (1 location per eye); the mid-vitreous (vitreous core) and posterior vitreous. A commercially made oxygen probe (Oxford Optronics) was used to measure the oxygen tension in various locations with respect to the oxygen delivery probe. For control experiments, the oxygen source was removed after the probe was positioned. After an hour in the dark, oxygen tension (pO2) recordings were performed using a micromanipulator to retract the probe to desired distances (0, 1, 2, 3, 4mm) away from the oxygen source. A two-sided, two sample t-test was conducted to statistically compare pO2 recordings.

 
Results
 

In the presence of an oxygen source, mid-vitreous pO2 and posterior vitreous pO2 measurements are higher across all distances as compared to the control pO2 measurements (N=4 for all groups). In the mid-vitreous experiments, there was a statistically significant difference between the oxygen source and control; (p<0.005) at all distances (0, 1, 2, 3, 4mm). In the posterior vitreous experiments, there was a statistically significant difference between oxygen source and control at all distances as well; 0mm (p<0.01), 1mm (p=0.01), 2mm (p<0.01), and 3mm (p=0.018) distances.<br /> Oxygen tension recordings are higher in the mid-vitreous compared to the posterior vitreous at all distances. The mid-vitreous oxygen tension is significantly different from the posterior vitreous oxygen tension at 2 and 3mm (p<0.001).

 
Conclusions
 

Our data demonstrates that vitreous oxygen consumption is higher in the posterior vitreous compared to the mid vitreous. This may be important in understanding the role of vitreous cortex and vitreous core in oxygen homeostasis. These findings also have important implications in the modeling of oxygen consumption in the vitreous and eye.  

 
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