June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Ca2+ signaling in perivascular cells during PGE2-induced relaxation and PGF-induced contraction of porcine retinal arterioles in vitro
Author Affiliations & Notes
  • Olga Kudryavtseva
    Ophthalmology, Aarhus University Hospital, Aarhus, Denmark
  • Toke Bek
    Ophthalmology, Aarhus University Hospital, Aarhus, Denmark
  • Footnotes
    Commercial Relationships Olga Kudryavtseva, None; Toke Bek, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4418. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Olga Kudryavtseva, Toke Bek; Ca2+ signaling in perivascular cells during PGE2-induced relaxation and PGF-induced contraction of porcine retinal arterioles in vitro . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4418.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Recently, a novel population of perivascular cells (PVC) located immediately external to the vascular smooth muscle cells of the retinal arterioles has been identified. PVCs may contribute to agonist-induced modulation of retinal arterioles tone, and the purpose of the present study was to identify the source of Ca2+ recruitment in the perivascular cells during PGE2-induced relaxation and PGF-induced contraction of porcine retinal arterioles.

Methods: Porcine retinal arterioles with preserved perivascular retinal tissue were mounted in a confocal myograph and loaded with the Ca2+-sensitive fluorophore Oregon Green. After either pre-constriction with U46619 (10-6 M) followed by the addition of the relaxing prostaglandin PGE2 (10-5 M) or addition of the constricting prostaglandin PGF (10-5 M) arteriolar tone and fluorescence from PVCs were recorded simultaneously with and without the presence of Ca2+ channel blockers (ryanodine, nifedipine, LOE908, CPA, 2-APB).

Results: The addition of PGE2 induced arteriolar relaxation and none of the Ca2+ channel blockers caused significant changes in this relaxation. Both PGE2 and PGF increased Ca2+ activity in the PVCs. CPA and 2-APB reduced the number of PVCs displaying Ca2+ activity after stimulation with both compounds, whereas ryanodine, nifedipine and LOE908 reduced calcium acitivity in PVCs stimulated with PGF.

Conclusions: PGE2 and PGFinduce Ca2+ activity in perivascular cells of porcine retinal arterioles, and sarcoplasmic reticulum and inositol triphosphate receptors are important sources of this Ca2+ signal. A coupling between PGE2-induced relaxation and Ca2+ recruitment in perivascular retinal cells is uncertain.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×