June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Effect of FP receptor and endogenous PG production against transient ocular hypertension induced by PGE2 and PGF in mouse eyes.
Author Affiliations & Notes
  • Reiko Yamagishi
    Dept of Ophthalmology Sch of Med, University of Tokyo, Bunkyo-ku, Japan
  • Makoto Aihara
    Shirato Eye Clinic, Tokyo, Japan
    Opthalmology & Visual Science, Tokyo Medical and Dental University, Tokyo, Japan
  • Footnotes
    Commercial Relationships Reiko Yamagishi, None; Makoto Aihara, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4419. doi:
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      Reiko Yamagishi, Makoto Aihara; Effect of FP receptor and endogenous PG production against transient ocular hypertension induced by PGE2 and PGF in mouse eyes.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4419.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We previously reported that PGE2 and PGF induced transient ocular hypertension in mouse eyes. However, the mechanism has not been clarified. In this study, we investigated the possible implication of FP receptor and endogenous PGs for transient ocular hypertension by PGE2 and PGF.

Methods: Intraocular pressure (IOP) was measured at day time using micro needle method in mouse eyes. A single drop with 3 μL aliquots of saline or 0.1% nepafenac solution (NEP), one of the NSAIDs, were topically applied into randomly selected one of two eyes in C57BL6 (WT), FP, EP1, EP2 and EP3 knock-out (KO) mice. After 30min, 0.1%PGE2 methyl ester (=PGE2) or PGF methyl ester (=PGF) were additionally dropped, and IOP was measured 60min later.<br /> IOP change was evaluated by the difference between IOP of the treated eye (IOPtx) and that of the contralateral control eye (IOPc) and the percentage of IOP inrease was calculated by 100x(IOPtx - IOPc)/IOPc in each mouse.

Results: IOP increase by PGE2 in WT, and FP, EP1, EP2 and EP3KO mice were 7.5±5.3, 27.9±11.6, 7.9±5.6, 8.7±5.5 and 6.2±6.0%, respectively. Conversely, those by NEP+PGE2 showed 4.8±6.6, 5.6±4.5, 10.5±8.3, 8.0±8.7 and 11.7±6.4%, respectively. Further, IOP increase by PGF were 8.9±6.6, 16.9±5.1, 6.4±5.5, 4.6±9.1 and 1.4±7.6%, respectively. In contrast, NEP+PGF showed 5.0±7.8, 2.5±3.0, 6.6±6.3, 5.5±5.2 and 3.5±2.1%, respectively. Transient ocular hypertension was induced in all types of mice by PGE2 and PGF, but was significantly enhanced in FPKO mouse compared to the other mice. (p<0.01) Moreover, the enhancement of ocular hypertension was significantly suppressed by NEP treatment. (p<0.01)

Conclusions: It became clear that transient ocular hypertension induced by PGE2 and PGF in mouse eyes might involve FP receptor and endogenous PG production.

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