June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Spectral counting-based LC-MS/MS quantitation of tear film proteins in ocular surface disease
Author Affiliations & Notes
  • Javier Soria Esponera
    Bioftalmik Applied Research, Derio, Spain
  • Arantxa Acera
    Bioftalmik Applied Research, Derio, Spain
  • Juan A Duran
    Instituto Clínico Quirúrgico de Oftalmología (ICQO), Bilbao, Spain
    Department of Ophthalmology, School of Medicine, University of the Basque Country (UPV/EHU), Leioa, Spain
  • Jesus Merayo
    Fernández Vega Ophthalmological Institute, Oviedo, Spain
  • Nerea Gonzalez
    Bioftalmik Applied Research, Derio, Spain
  • Tatiana Maria Suarez-Cortes
    Bioftalmik Applied Research, Derio, Spain
  • Footnotes
    Commercial Relationships Javier Soria Esponera, None; Arantxa Acera, None; Juan Duran, None; Jesus Merayo, None; Nerea Gonzalez, None; Tatiana Suarez-Cortes, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4472. doi:
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      Javier Soria Esponera, Arantxa Acera, Juan A Duran, Jesus Merayo, Nerea Gonzalez, Tatiana Maria Suarez-Cortes, Bioftalmik Applied Research; Spectral counting-based LC-MS/MS quantitation of tear film proteins in ocular surface disease. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4472.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Dry eye (DE) and meibomian gland dysfunction (MGD) are the most common ocular surface diseases. Discrimination between these conditions is difficult because of the overlapping signs and symptoms and the lack of correlation between these symptoms and clinical parameters. We searched for the differences between tear proteomes in these two conditions.

 
Methods
 

Tear samples were collected from 37 individuals: 7 with DE disease, 12 with MGD, and 18 control subjects. Diagnosis was based on clinical examination including the Schirmer test with anesthesia to measure only the basal secretion, slit-lamp examination of the lid margin and meibomian glands, fluorescein staining, and subjective symptoms. Each patient answered a modified “National Eye Institute Visual Functioning Questionnaire 25” (VFQ-25), which included some statements about problems with their vision or wellbeing .<br /> The samples were analyzed using spectral counting-based LC-MS/MS quantitation.

 
Results
 

Comparative analysis of tear protein profiles revealed discriminating changes in the expression levels of 26 proteins, including protein S100A6, annexin A1, cystatin-S, thioredoxin, phospholipase A2, antileukoproteinase, and lactoperoxidase. Some of the proteins with altered expression have been reported and validated previously. Comparative studies conducted using other proteomics technologies have already discovered some tear biomarkers for DE and MGD.

 
Conclusions
 

Our study confirmed S100A6, cystatin-S, annexin A1, and phospholipase A2 as valid tear biomarkers for these conditions. Subsequent functional network analysis revealed the main biological processes that should be examined to improve our understanding of ocular surface diseases and identify new candidate therapeutic targets.  

 
 
Canonical Discriminant Analysis showing the separation between the samples using APEX expression data. Each of the points represents a sample from each group. Good separation between the groups is apparent. The MGD group was closer to the control group (CT) than the DE group. Key: squares, CT group; triangles, MGD group; circles, DE group.
 
Canonical Discriminant Analysis showing the separation between the samples using APEX expression data. Each of the points represents a sample from each group. Good separation between the groups is apparent. The MGD group was closer to the control group (CT) than the DE group. Key: squares, CT group; triangles, MGD group; circles, DE group.

 
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