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Takayoshi Sumioka, Yuka Okada, Ken-ichi Matsumoto, Osamu Yamanaka, Masayasu Miyajima, Shizuya Saika; Loss of tenascin X suppresses expression of VEGF in macrophages and of TGFb1 in ocular fibroblasts in vitro; possible mechanism of inhibition of neovascularization in cornea. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4498.
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© ARVO (1962-2015); The Authors (2016-present)
To examine mRNA expression level of angiogenic growth factors, VEGF and TGFb1, in macrophages and ocular fibroblasts derived from a tenascin X-null mouse. We previously reported that the loss of tenascin X suppress neocvascularization with reduction of in vivo expression of angiogenic growth factors in a mouse cornea (ARVO 2014).
Peritoneal macrophages were obtained from tenascin X-null and wild types mice by using macrophage induction by oyster glycogen i.p. injection. Ocular fibroblasts were cultured from eye-shells of post-natal day 1 or 2 mice. The cultures were maintained for 24 hrs with or without exogenous TGFb1 and processed for RNA extraction. Real-time RT-PCR was ran to examine the expression level of VEGF and TGFb1.
Loss of tenascin X supprsss mRNA expression of VEGF in macrophages and of TGFb1 in fibroblasts in the absence of TGFb1. Loss of tenascin X did not attenuate the TGFb1 induction of VEGF and TGFb1 in these cell types.
Tenascin X is involved in expression of angiogenic growth factors in macrophages and fibroblasts, partially explain the mechanism of inhibition of in vivo neovascularization in a tenacin X-null mouse cornea.
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