June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Adaptive optics imaging of putative cone inner segments within geographic atrophy lesions
Author Affiliations & Notes
  • Ethan A Rossi
    Center for Visual Science, University of Rochester, Rochester, NY
  • Kenichi Saito
    Canon U.S.A., Inc., Melville, NY
  • Charles E. Granger
    The Institute of Optics, University of Rochester, Rochester, NY
  • Koji Nozato
    Canon U.S.A., Inc., Melville, NY
  • Qiang Yang
    Center for Visual Science, University of Rochester, Rochester, NY
  • Tomoaki Kawakami
    Canon, Inc., Tokyo, Japan
  • Jie Zhang
    Center for Visual Science, University of Rochester, Rochester, NY
  • William Fischer
    Flaum Eye Institute, University of Rochester Medical Center, Rochester, NY
  • David R Williams
    Center for Visual Science, University of Rochester, Rochester, NY
    The Institute of Optics, University of Rochester, Rochester, NY
  • Mina M Chung
    Flaum Eye Institute, University of Rochester Medical Center, Rochester, NY
  • Footnotes
    Commercial Relationships Ethan Rossi, University of Rochester (P); Kenichi Saito, Canon U.S.A., Inc. (E), Canon, Inc. (F), Canon, Inc. (P), University of Rochester (P); Charles Granger, Canon, Inc. (F); Koji Nozato, Canon U.S.A., Inc. (E), Canon, Inc. (F), Canon, Inc. (P), University of Rochester (P); Qiang Yang, Canon, Inc. (F), Canon, Inc. (P), Montana State University (P), University of Rochester (P); Tomoaki Kawakami, Canon, Inc. (E), Canon, Inc. (P); Jie Zhang, Canon, Inc. (F), Canon, Inc. (P), University of Rochester (P); William Fischer, Canon, Inc. (F); David Williams, Canon, Inc. (F), Canon, Inc. (R), University of Rochester (P), University of Rochester (P); Mina Chung, Canon, Inc. (F), GlaxoSmithKline (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4931. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ethan A Rossi, Kenichi Saito, Charles E. Granger, Koji Nozato, Qiang Yang, Tomoaki Kawakami, Jie Zhang, William Fischer, David R Williams, Mina M Chung; Adaptive optics imaging of putative cone inner segments within geographic atrophy lesions. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4931.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

The time course of cone loss relative to the formation of geographic atrophy (GA) lesions in age-related macular degeneration is not well characterized. Confocal adaptive optics scanning light ophthalmoscopy (AOSLO) images of GA lesions contain hyper-reflective cone-like features, but such images are difficult to interpret in advanced disease states such as GA. Non-confocal split-detector imaging in AOSLO has been shown to reveal structures consistent with cone inner segments (Scoles et al. 2014). Here we use two non-confocal AOSLO methods to determine whether putative cone inner segments exist within GA lesions and if they correlate with the cone-like structures seen in confocal AOSLO images.

 
Methods
 

Six patients were imaged, five with near-infrared (NIR) light in a compact commercial AOSLO prototype modified for split-detector imaging. The central portion of the PSF was directed to one detector for confocal imaging and a knife edge prism split the remaining light into two additional detectors. Five patients were imaged with visible light in a research AOSLO with a novel non-confocal method based on offset aperture (Chui et al. 2012) and split-detector methods. At each location, images were sequentially obtained with a ~10 Airy disk aperture offset by ~6 Airy disks to 8 equally spaced radial positions. Images were co-registered with simultaneously acquired NIR images. Registered images from each position were averaged and combined to enhance contrast. Identical locations were imaged in both instruments in some eyes permitting method comparison.

 
Results
 

Structures consistent in appearance to putative cone inner segments were observed within some small GA lesions. Cone-like features in confocal AOSLO images did not always co-localize with putative cone inner segments, suggesting that some hyper-reflective features in confocal AOSLO arise from light scattered by other structures. Conversely, some areas where cones were not visible in confocal AOSLO contained putative cone inner segment structures in non-confocal images.

 
Conclusions
 

Some cones survive in small GA lesions. Images similar to those obtained with the split-detector method can be obtained using a single detector. Cone survival in small GA lesions offers hope that future treatments aimed at restoring RPE health may rescue remaining cones. Further study is needed to understand the rate of cone survival after RPE cell loss is initiated in AMD.

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×