June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Are the dyes enough for a complete peeling of the ILM or ERM and to distinguish the causes of the ERM ? : histopathological study.Up to Date.
Author Affiliations & Notes
  • Matias Iglicki
    Hospital de Clinicas, Caba, Argentina
  • jorge zarate
    Hospital de Clinicas, Caba, Argentina
  • Lucia Comastri
    Hospital de Clinicas, Caba, Argentina
  • juan cortalezzi
    Hospital de Clinicas, Caba, Argentina
  • juan pablo francos
    Hospital de Clinicas, Caba, Argentina
  • Diego A Bar
    Hospital de Clinicas, Caba, Argentina
  • Carmen N Demetrio
    Hospital de Clinicas, Caba, Argentina
  • pablo chiaradia
    Hospital de Clinicas, Caba, Argentina
  • Marcelo Zas
    Hospital de Clinicas, Caba, Argentina
  • Footnotes
    Commercial Relationships Matias Iglicki, None; jorge zarate, None; Lucia Comastri, None; juan cortalezzi, None; juan pablo francos, None; Diego Bar, None; Carmen Demetrio, None; pablo chiaradia, None; Marcelo Zas, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5057. doi:
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      Matias Iglicki, jorge zarate, Lucia Comastri, juan cortalezzi, juan pablo francos, Diego A Bar, Carmen N Demetrio, pablo chiaradia, Marcelo Zas; Are the dyes enough for a complete peeling of the ILM or ERM and to distinguish the causes of the ERM ? : histopathological study.Up to Date. . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5057.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To evaluate whether it is necessary to do a histophathological study of the tissues which were peeled off from the epiretinal membrane or macular hole or whether the use of dyes (like Brilliant Blue and Trypan Blue) is enough.Also to realize if using dyes we can identified the causes of the ERM ( primary, secondary or idiopathic)

 
Methods
 

Fifty different patients with vitreoretinal interface pathologies, such as epiretinal membrane and macular holes, were operated on using the 23G vitrectomy technique in epiretinal membrane surgery. Trypan blue dye was used for staining of the ERM and brilliant blue G dye was used to stain the ILM. The tissue removed during the vitrectomy was sent to the Anatomical Pathology Department to undergo histopathological analysis. The samples were labelled with a code, so as the pathologist would not know which tissue was being analysed. The diagnosis and monitoring of patients were done by means of: symptoms, fluorescein angiography, Optical Coherence Tomography (OCT) and Best Corrected Visual Acuity (BCVA) with ETDRS charts before and after surgery. The patients’ visual acuity prior to surgery was 20/200 and it increased to 20/80 after surgery. Patients were monitored through Visual Acuity and OCT every three months after surgery.

 
Results
 

The anatomopathological results indicated that 100% of the sample tissues which were considered to be ILM or ERM were in fact correct.Also the pathologist was able to distinguish the cause of ERM, i.e. primary secondary( inflammatory) or idiopathic

 
Conclusions
 

Based on the results thrown by this study, it can be concluded that both, brilliant blue G and Trypan blue, are excellent anatomical markers for the removal of the epiretinal membrane as well as the ILM. In consequence, the histopathological analysis would not be a necessary step.<br /> It can be added that using dyes we can distinguish several cells markers that tell us the cause of the ERM.we can diagnose diseases such as diabetes even before that the patients have any sing or symptom.  

 

 
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