June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Comparison of serum and vitreous PIGF in diabetic retinopathy patients and non-diabetic patients
Author Affiliations & Notes
  • Joana Mesquita
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • João Paulo Castro Sousa
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
    Ophthalmology, Centro Hospitalar de Leiria, Leiria, Portugal
  • Paulo Tavares-Ratado
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • Sara Vaz-Pereira
    Ophthalmology, Hospital de Santa Maria, Lisbon, Portugal
    Faculty of Medicine, University of Lisbon, Lisbon, Portugal
  • Arminda Neves
    Ophthalmology, Centro Hospitalar de Leiria, Leiria, Portugal
  • Ana S. Rocha
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • Fátima Santos
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • Luís Passarinha
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • Candida Tomaz
    CICS-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
  • Footnotes
    Commercial Relationships Joana Mesquita, Alimera Sciences (E); João Paulo Castro Sousa, None; Paulo Tavares-Ratado, None; Sara Vaz-Pereira, None; Arminda Neves, None; Ana Rocha, None; Fátima Santos, None; Luís Passarinha, None; Candida Tomaz, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5179. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Joana Mesquita, João Paulo Castro Sousa, Paulo Tavares-Ratado, Sara Vaz-Pereira, Arminda Neves, Ana S. Rocha, Fátima Santos, Luís Passarinha, Candida Tomaz; Comparison of serum and vitreous PIGF in diabetic retinopathy patients and non-diabetic patients. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5179.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. The exact role of PlGF in vascular development is yet to be established. PIGF binds to VEGFR-1 and potentiates VEGF-A angiogenic activity. The role of PlGF is restricted to pathological conditions and is therefore a possible target for therapy. PIGF could be used for therapeutic angiogenesis of ischemic cardiovascular disease, but inhibition of PlGF also suppresses pathological angiogenesis in inflammatory disorders and diabetic retinas. The aim of this study was to evaluate serum and vitreous PIGF levels by ELISA in diabetic patients (with diabetic retinopathy (DR)) vs. non-diabetic patients (with rhegmatogenous retinal detachment (RRD)).

 
Methods
 

Serum and vitreous samples were collected from 38 patients undergoing vitrectomy. ELISA was used to quantify vitreous and serum levels of PIGF. All patients included in this study, which adhered to the tenets of the Declaration of Helsinki, gave their informed consent to surgical treatment.

 
Results
 

Seventeen patients were non-diabetic and twenty-one were diabetic. In the diabetic group, four had non-proliferative DR (NPDR) and seventeen had proliferative DR (PDR). Mean vitreous PIGF concentration was significantly higher for diabetic patients when compared to non-diabetic patients (70.0 vs. 46.47 pg/ml, Z = -2,847, p = 0.004). Mean serum PIGF concentration was also higher in diabetic patients when compared to non-diabetic patients (50.5 vs. 48.8 pg/ml), although not statistically significant (Z = -1,196, p = 0.232). Comparison between DR group, showed that PDR patients had significantly higher vitreous levels of PIGF vs. NPDR patients, (76.5 vs. 42.5 pg/ml, Z = -2.612, p = 0.009).

 
Conclusions
 

PIGF is overexpressed in the vitreous of diabetic patients and levels may increase with the severity of the disease. We could not conclude in this study about a correlation between vitreous and serum levels of PIGF.  

 

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×