Purchase this article with an account.
Joana Mesquita, João Paulo Castro Sousa, Paulo Tavares-Ratado, Sara Vaz-Pereira, Arminda Neves, Ana S. Rocha, Fátima Santos, Luís Passarinha, Candida Tomaz; Comparison of serum and vitreous PIGF in diabetic retinopathy patients and non-diabetic patients. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5179.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. The exact role of PlGF in vascular development is yet to be established. PIGF binds to VEGFR-1 and potentiates VEGF-A angiogenic activity. The role of PlGF is restricted to pathological conditions and is therefore a possible target for therapy. PIGF could be used for therapeutic angiogenesis of ischemic cardiovascular disease, but inhibition of PlGF also suppresses pathological angiogenesis in inflammatory disorders and diabetic retinas. The aim of this study was to evaluate serum and vitreous PIGF levels by ELISA in diabetic patients (with diabetic retinopathy (DR)) vs. non-diabetic patients (with rhegmatogenous retinal detachment (RRD)).
Serum and vitreous samples were collected from 38 patients undergoing vitrectomy. ELISA was used to quantify vitreous and serum levels of PIGF. All patients included in this study, which adhered to the tenets of the Declaration of Helsinki, gave their informed consent to surgical treatment.
Seventeen patients were non-diabetic and twenty-one were diabetic. In the diabetic group, four had non-proliferative DR (NPDR) and seventeen had proliferative DR (PDR). Mean vitreous PIGF concentration was significantly higher for diabetic patients when compared to non-diabetic patients (70.0 vs. 46.47 pg/ml, Z = -2,847, p = 0.004). Mean serum PIGF concentration was also higher in diabetic patients when compared to non-diabetic patients (50.5 vs. 48.8 pg/ml), although not statistically significant (Z = -1,196, p = 0.232). Comparison between DR group, showed that PDR patients had significantly higher vitreous levels of PIGF vs. NPDR patients, (76.5 vs. 42.5 pg/ml, Z = -2.612, p = 0.009).
PIGF is overexpressed in the vitreous of diabetic patients and levels may increase with the severity of the disease. We could not conclude in this study about a correlation between vitreous and serum levels of PIGF.
This PDF is available to Subscribers Only