June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Imaging of Periarteriolar Capillary Free Zone using Offset Pinhole Adaptive Optics Scanning Light Ophthalmoscopy
Author Affiliations & Notes
  • Toco Yuen Ping Chui
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Nikhil Menon
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Nadim Choudhury
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Alexander Pinhas
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Michael Dubow
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Nishit Shah
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
  • Alfredo Dubra
    Ophthalmology, Medical College of Wisconsin, Milwaukee, WI
    Biophysics, Medical College of Wisconsin, Milwaukee, WI
  • Richard B Rosen
    Ophthalmology, New York Eye & Ear Infirmary of Mount Sinai, New York, NY
    Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, NY
  • Footnotes
    Commercial Relationships Toco Chui, None; Nikhil Menon, None; Nadim Choudhury, None; Alexander Pinhas, None; Michael Dubow, None; Nishit Shah, None; Alfredo Dubra, Canon USA Inc (C), US patent 8,226,236 (P); Richard Rosen, Advanced Cellular Technologies (C), Advanced Cellulat Technologies (C), Allergan (C), Clarity (C), OD-OS (C), Opticology (I), Optovue (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5298. doi:
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      Toco Yuen Ping Chui, Nikhil Menon, Nadim Choudhury, Alexander Pinhas, Michael Dubow, Nishit Shah, Alfredo Dubra, Richard B Rosen; Imaging of Periarteriolar Capillary Free Zone using Offset Pinhole Adaptive Optics Scanning Light Ophthalmoscopy. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5298.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Previous studies have shown that the dimension of the periarteriolar capillary free zone (CFZ) is associated with the retinal oxygen level. In this study, we imaged and quantified the CFZ in healthy controls using an offset pinhole adaptive optics scanning light ophthalmoscope (AOSLO).

Methods: Periarteriolar CFZ imaging was performed on 7 healthy controls (7 eyes, 6 males) using an offset pinhole AOSLO with an imaging wavelength centered at 790nm. Images were obtained along the retinal vascular arcades starting at the superior or inferior optic disc margin. Image sequences were acquired using a 1.5-degree field of view at a frame rate of 15Hz. After sinusoidal distortion and eye motion artifacts were removed, averaged vascular structural images and motion contrast vascular perfusion maps were obtained for quantitative measurements of lumen diameter, vessel wall thickness, and periarteriolar CFZ width using a semi-automatic MATLAB software.

Results: Arteriolar lumen diameter, vessel wall thickness, and CFZ width were visualized in all subjects. CFZ was found at the same retinal depth as the arterioles. Vessels with a lumen diameters ranging in size from 16 to 76 µm were imaged and measured. The mean±SD µm of lumen diameter, vessel wall thickness, and CFZ was 42±15 µm, 10±2 µm, and 46±7 µm, respectively. The wall-to-lumen ratio was 0.24±0.08 µm (range: 0.13-0.44). The CFZ width measurements ranged from 36 to 71 µm. Although there was a weak positive correlation, no statistical significance was found between the CFZ width and lumen diameter (Linear Regression; p>0.05).

Conclusions: Offset pinhole AOSLO provides the ability to noninvasively and quantitatively assess periarteriolar CFZ. This has the potential for better understanding of the retinal vascular development in the healthy retina and in disease states such as diabetic retinopathy and retinopathy of prematurity.

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