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Arlene V Drack, Uma Balakrishnan, Andrew Kemerley, Nicole Tatro, Alina Valentina Dumitrescu, Stewart Thompson, Sajag Bhattarai; Effect of operating microscope light on retinal function in common laboratory mice strains. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5415.
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© ARVO (1962-2015); The Authors (2016-present)
Light has been noted to be a co-factor for progression of retinal degeneration in some animal models of retinitis pigmentosa(RP). Human patients with RP are advised to avoid bright light and concerns about retinal toxicity from ophthalmic operating microscope light(OML) have been raised for mice and humans with retinal degenerations. We sought to determine the effect of exposure to OML on retinal function in mice.
Mice from multiple retinal disease strains received ketamine/xylazine anesthesia and eyes were exposed to light (32mW/cm2) from the operating microscope (Zeiss, West Germany) by (1) dilating right eyes and positioning mice left side down with left eye occluded under the microscope for 35 minutes on 3 consecutive days. (2) Mice of the same age and strain were divided into experimental and control groups. On 3 consecutive days the experimental group was anesthetized, dilated and exposed to the OML focused equidistant between the eyes for 35 minutes. In a separate experiment, rd10 mice were reared in darkness or standard light/dark cycles. A subset of dark-reared rd10 were exposed to bright external light (9mW/cm2) while awake 40 minutes a day for 4 days. For all experiments, electroretinogram(ERG) was performed one week after final light exposure and at pre-determined time points thereafter.
The following mice strains showed no statistically significant difference in ERG amplitudes between eyes after unilateral OML exposure: Bbs1M390R/M390R (n=4), rd10 (n=4), rd12(n=3), 129SVEV WT(n=3), albino(n=4). For mice with bilateral OML exposure the following strains showed no difference between exposed and unexposed mice: B6 WT(n=14), 129SVEV WT(n=4), Bbs1(n=4). Dark-reared rd10 mice (n=15) showed significantly higher ERG amplitudes than light cycled mice (n=7) (p=0.0002). Dark-reared rd10 briefly exposed to bright external light had reduced ERG amplitudes compared to dark-reared unexposed(p=3E-11).
Despite the fact that light intensifies retinal degeneration in some animal models of RP, brief repeated exposure to OML does not adversely affect ERG amplitudes short term in many laboratory mouse models reared in typical cyclic light/dark. Dark-reared rd10 have significantly higher ERG amplitudes than cyclic light reared; this can be abolished by short term exposure to bright light.<br /> <br /> References: Komaromy, Acland, Aguirre. Arch Ophthalmol.2008;126(5):714-717.
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