June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Functional and morphological alterations of older rhodopsin P347L transgenic rabbits
Author Affiliations & Notes
  • Taro Kominami
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
    Ophthalmology, Anjo Kosei Hospital, Anjo, Japan
  • Shinji Ueno
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
  • Rika Hirota
    Astellas Pharma Inc., Osaka, Japan
  • Azusa Kominami
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
  • Ayami Nakanishi
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
  • Masatoshi Nagaya
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
  • Mineo Kondo
    Ophthalmology, Mie Univ School of Med, Tsu, Japan
  • Hiroko Terasaki
    Ophthalmology, Nagoya Univ School of Med, Nagoya, Japan
  • Footnotes
    Commercial Relationships Taro Kominami, None; Shinji Ueno, None; Rika Hirota, None; Azusa Kominami, None; Ayami Nakanishi, None; Masatoshi Nagaya, None; Mineo Kondo, None; Hiroko Terasaki, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5433. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Taro Kominami, Shinji Ueno, Rika Hirota, Azusa Kominami, Ayami Nakanishi, Masatoshi Nagaya, Mineo Kondo, Hiroko Terasaki; Functional and morphological alterations of older rhodopsin P347L transgenic rabbits. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5433.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: A rhodopsin P347L transgenic (Tg) rabbit, a model of retinitis pigmentosa, has been generated in our laboratory. We have reported the functional and morphological alterations of this animal until 12-months-of-age (Kondo M et al. 2009, IOVS). The purpose of this study was to evaluate the functional and morphological changes of older Tg rabbits.

Methods: We studied 25 Tg and 5 wild type (WT) rabbits of New Zealand White background whose ages ranged from 18 to 60 months. Full-field ERGs were recorded to determine when the ERGs became non-recordable in Tg rabbits. Spectral-domain optical coherent tomography (SD-OCT) was used to measure the retinal thickness of 6 Tg rabbits and 5 WT rabbits at 3-years-of-age. The histological changes of the retina in Tg and WT rabbits were examined by light and electron microscopy after the ERGs became non-recordable.

Results: The ERGs of Tg rabbits became non-recordable between 30 and 54 months, and all were non-recordable by 54-months-of-age. SD-OCT showed that the retinal thickness was not reduced in the Tg rabbits at 3-years-of-age in spite of the photoreceptor degeneration. This indicated a thickening of the inner retina in these Tg rabbits. Histological analysis of the Tg rabbits after the ERGs became non-recordable showed that the photoreceptors were totally degenerated. However, the structure of the inner nuclear and ganglion cell layers were highly disorganized with a proliferation of glial cells.

Conclusions: Our results showed that it took less than 54 months for the ERGs to be non-recordable in this Tg rabbit. We found that retinal remodeling with glial cell proliferation was one of the features of this rabbit model at an advanced stage of retinal degeneration.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×