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Yukihisa Takada, Osamu Yamanaka, Takayoshi Sumioka, Yuka Okada, Shizuya Saika; Travoprost promotes EGF expression in cultured corneal epithelial cells and alters proliferation and E-cadherin expression in epithelium of an organ-cultured mouse cornea.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5630.
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© ARVO (1962-2015); The Authors (2016-present)
To examine the effects of travoprost on the expression of epidermal growth factor (EGF) in cultured human corneal epithelial cells (HCE) and expression pattern of E-cadherin and Ki67 in epithelium of an organ-cultured mouse cornea. We previously reported that Travatanz® and the ingredient, travoprost, both diluted X100, promotes spreading of corneal epithelium in organ-culture and HCE proliferation (ARVO 2011, 2012).
Confluent HCE culture was treated with travoprost at the concentration equivalent to travatanz® (Alcon) x100 diluted. mRNA expression of EGF, TGFb1 and VEGF was examined by real-time RT-PCR. Eye globes of C57/BL6 mice were cultured in serum-free medium in the presence of travoprost at the concentration same as above or EGF (10 ng/ml) for 48 hrs. Expression pattern of E-cadherin and Ki67 was observed by using immunohistochemistry.
Travoprost increased mRNA expression of EGF, but not TGFb1 and VEGF. Adding either travoprost or EGF promoted KI67 expression in basal epithelial cells and also altered the expression pattern of E-cadherin in corneal epithelium; E-cadherin was faintly detected in cell-cell contact in corneal epithelium, but travoprost or EGF increased E-cadherin immunoreactivity in the cytoplasm of epithelial cells.
Travoprost accelerates EGF expression in corneal epithelial cells and affects E-cadherin localization and cell proliferation. Further study is needed to examine if these effects of travoprost are involved in its toxic effects on corneal epithelium.
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