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Peter S. Reinach, Dongsheng Yan, Weiwei Xu, Lili Tu; microRNA-184 Downregulation Contributes to Corneal Epithelial Wound Healing . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5657.
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microRNAs (miRNAs) are endogenous short (~22) nucleotide non-coding RNAs which inhibit protein translation through binding to target mRNAs. Recent studies have demonstrated that miR-184 can inhibit tumor cell proliferation and migration. Its role in corneal epithelial renewal, however, remains largely unknown. In the present study, we investigated the function of miR-184 in corneal epithelial wound healing.
Realtime RT-PCR was performed to detect the expression of miR-184 in mouse corneal epithelium during the wound healing process. Human corneal epithelial cells were transfected with miR-184 using Lipofectamine RNAiMAX reagent. MTS and a wound-healing assay were carried out to evaluate the effects of miR-184 on human corneal epithelial cell proliferation and migration. Flow cytometry evaluated cell cycle progression.
miR-184 was dramatically downregulated in corneal epithelial wound healing. Transfection of miR-184 into human corneal epithelial cells led to a significant decrease in cell proliferation and induced cell cycle G2-arrest. Furthermore, miR-184 transfection inhibited cell migration.
Our results demonstrate that miR-184 inhibits human corneal epithelial cell proliferation and migration. This indicates that miR-184 downregulation contributes to hastening these responses during corneal epithelial wound healing.
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