June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Improvement of hyper reflective dots visualization on OCT by gold nanoparticles
Author Affiliations & Notes
  • Patricia Fernandez-Robredo
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
  • Michael Powner
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
  • Senthil Selvam
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
  • Dawn A Sim
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
    NIHR Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
  • Pearse Andrew Keane
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
    NIHR Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
  • Marcus Fruttiger
    Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom
  • Footnotes
    Commercial Relationships Patricia Fernandez-Robredo, None; Michael Powner, None; Senthil Selvam, None; Dawn Sim, None; Pearse Keane, None; Marcus Fruttiger, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5925. doi:
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      Patricia Fernandez-Robredo, Michael Powner, Senthil Selvam, Dawn A Sim, Pearse Andrew Keane, Marcus Fruttiger; Improvement of hyper reflective dots visualization on OCT by gold nanoparticles. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5925.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: In previous work, we showed in a mouse model of choroidal neovascularisation (CNV) that circulating monocytes invading the retina can be labelled by indocyanine green (ICG) and imaged in vivo. Here we aimed to investigate the feasibility of using gold nanoparticles as contrast agents to label invading inflammatory cells on the OCT in the retina.

Methods: Gold nanoshells, nanorods, nanobypiramids and silver plates were pre-screened in a tissue phantom (agar sheets) to establish the best suitable contrast agent in OCT. Gold nanoshells (GNS) were selected for in vivo analyse in the mouse CNV model. Mice (n=5) were i.p. injected with GNS 3 days before the animals were lasered with a 532 nm diode laser (Micron III, Phoenix Research) and compared with control animals (n=5). Three to four laser spots were made close to the optic nerve in each eye. OCT images were taken on SD-OCT (Envisu R2200, Bioptigen), immediately after and 7 days post CNV induction. Fluorescein angiography was performed 7 days after laser. Animals were sacrificed, eyes flatmounted and subjected to lectin (IB4) staining and analysed using fluorescence microscopy.

Results: Hyper reflective dots (HRDs) were visible in OCT images one week after the CNV induction, surrounding CNV lesion area. Those dots were brighter in animals injected with nanoshells compared to controls. The HRD observed in the OCT correlated with lectin positive cells observed in the flatmount samples with macrophage-like morphology.

Conclusions: This study shows that gold nanoparticles can improve the visualisation of inflammatory cells on OCT. HRDs in OCT are observed clinically, but it has been difficult to prove what cells the HRDs exactly represent. Further studies are needed to characterise these cells.

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