June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Reproducibility of Macular Pigment Optical Density Measurement by Two-wave Length Autofluorescence
Author Affiliations & Notes
  • Qisheng You
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
    Beijng Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China
  • Dirk-Uwe G Bartsch
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
  • Mark Espina
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
  • Mostafa Alam
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
  • Natalia Camacho
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
  • William R Freeman
    Jacobs Retina Center, Shiley Eye Center, University of California San Diego, San Diego, CA
  • Footnotes
    Commercial Relationships Qisheng You, None; Dirk-Uwe Bartsch, None; Mark Espina, None; Mostafa Alam, None; Natalia Camacho, None; William Freeman, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5944. doi:
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    • Get Citation

      Qisheng You, Dirk-Uwe G Bartsch, Mark Espina, Mostafa Alam, Natalia Camacho, William R Freeman; Reproducibility of Macular Pigment Optical Density Measurement by Two-wave Length Autofluorescence. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5944.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Macular pigment, composed of lutein, zeaxanthin, and meso-zeaxanthin, is postulated to protect against age-related macular degeneration (AMD), likely due to filtering blue light and its antioxidant properties. It was reported that the macular pigment optical density (MPOD) is associated with macular function evaluated by visual acuity and electroretinogram. However, the reproducibility of measurement of MPOD, which is essential for any application purpose, has not yet been determined. The main purpose is to determine the reproducibility of MPOD measurement by two-wave length autofluorescence method.

 
Methods
 

Fifty four eyes of 31 persons were enrolled in the study, including 6 normal eyes, 15 wet AMD, 9 dry AMD, 8 macular edema due to diabetic macular, branch retinal vein occlusion or macular telangiectasia and 16 tractional maculopathy including vitreomacular traction, epiretinal membrane and macular hole. MPOD was measured with a two-wave length (488 and 514nm) autofluorescence method with Spectralis HRA+OCT after pupil dilation. The measurement was repeated for each eye 10 minutes later. The Pearson Correlation analysis and Bland-Altman plot were used to assess the reproducibility between the two measurements.

 
Results
 

The mean sum of MPOD volume within eccentricities of 1°, 2° and 6° radius was 906.61±440.84, 2332.57±1142.44, 5872.32±3394.97 and 893.74±430.40, 2302.69±1131.46, 5832.13±3416.64 at first and second measurement respectively. The correlation coefficient of the association (r) between the two measurements was 0.994, 0.994 and 0.988 (all P<0.001) respectively, and the Bland-Altman plot showed 9.3%, 3.7% and 5.6% points outside the 95% limits of agreement, indicating an overall excellent reproducibility.The data for the reproducibility did not differ markedly among the various disease and normal eyes.

 
Conclusions
 

Under routine examination conditions with pupil dilation, MPOD measurement by two-wave length autofluorescence method showed a high reproducibility.  

 
Scatter plot showing the correlation between the 1st and 2nd MPOD measurement within 1 degree radius of fovea
 
Scatter plot showing the correlation between the 1st and 2nd MPOD measurement within 1 degree radius of fovea
 
 
Bland-Altman plot of MPOD within 1 degree radius of the 1st and 2nd measurement. X-axial defined as mean of the 1st and 2nd measurement. Y-axial defined as the first measurement value minus the second measurement value. The mean differences and the 95% confidence limits of the bias are shown as three lines.
 
Bland-Altman plot of MPOD within 1 degree radius of the 1st and 2nd measurement. X-axial defined as mean of the 1st and 2nd measurement. Y-axial defined as the first measurement value minus the second measurement value. The mean differences and the 95% confidence limits of the bias are shown as three lines.

 
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