June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Repeatability and Reproducibility of Quantifying Parafoveal Vessel Density in Normal Subjects with OCT-based Microangiography
Author Affiliations & Notes
  • Zhongdi Chu
    Bioengineering, University of Washington, Seattle, WA
  • Qinqin Zhang
    Bioengineering, University of Washington, Seattle, WA
  • Chieh-Li Chen
    Bioengineering, University of Washington, Seattle, WA
  • Fengyi Luo
    Bioengineering, University of Washington, Seattle, WA
  • Cecilia Lee
    Ophthalmology, University of Washington, Seattle, WA
  • James L Kinyoun
    Ophthalmology, University of Washington, Seattle, WA
  • Ruikang K Wang
    Bioengineering, University of Washington, Seattle, WA
    Ophthalmology, University of Washington, Seattle, WA
  • Footnotes
    Commercial Relationships Zhongdi Chu, None; Qinqin Zhang, None; Chieh-Li Chen, None; Fengyi Luo, None; Cecilia Lee, None; James Kinyoun, None; Ruikang Wang, Carl Zeiss Meditec (F), Carl Zeiss Meditec (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5958. doi:
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      Zhongdi Chu, Qinqin Zhang, Chieh-Li Chen, Fengyi Luo, Cecilia Lee, James L Kinyoun, Ruikang K Wang; Repeatability and Reproducibility of Quantifying Parafoveal Vessel Density in Normal Subjects with OCT-based Microangiography. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5958.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To demonstrate the reliability of OCT-based microangiography (OMAG) for non-invasive, label-free in vivo imaging of the retinal microvasculature in macular region of normal subjects and for the measurement of vessel density in central fovea and parafoveal quadrants.

 
Methods
 

A prospective, observational study was conducted with ten normal subjects (twenty eyes; age = 30.9±7.17). Each subject was scanned three times per eye with a Cirrus HD-5000 OCT-angiography prototype using OMAG scanning protocol. The retinal microcirculation was depicted as an en face image. Two masked graders performed segmentation of the resulting OMAG images, outlined the foveal avascular zone (FAZ) with ellipse or free-hand drawing approach and performed independent vessel density quantification. The parafoveal and foveal regions were outlined with circles of diameters of 2.5mm and 1.5mm; vessel density was calculated for central foveal region (FAZ excluded) and in quadrants for parafovea. Descriptive statistics, repeatability and reproducibility were calculated.

 
Results
 

The vessel density of defined sub-field varied among subjects (range of 0.136-0.288). The mean values of each sub-field were 0.223±0.022 for parafoveal superior quadrant, 0.22±0.022 for parafoveal inferior quadrant, 0.234±0.022 for parafoveal nasal quadrant, 0.23±0.024 for parafoveal temporal quadrant and 0.222±0.02 for central fovea. There were no statistically significant differences among four parafoveal quadrants, but statistically significant differences were observed between parafoveal nasal and central foveal regions (p = 3e-5) as well as between parafoveal temporal and central foveal regions (p = 0.0149). The measurements were reliable: the repeatability (%) was 9.7% (Sr = 0.021), the reproducibility (%) was 5.3% (SR = 0.012), and the intra-class coefficient (ICC) was 0.96 while the coefficient of variance was 5.4%.

 
Conclusions
 

OMAG is a noninvasive 3D imaging technique that can provide depth-resolved retinal microvasculature information with capillary level resolution in real-time. Blood flow distribution in macular region can be easily identified and allow repeatable and reproducible parafoveal vessel density measurements.  

 
Fig.1 Vessel density quantification. (a) Illustration of vessel density measurement in defined sub-fields; (b) Box-and-whisker plot of vessel density measurement and correspongding p value.
 
Fig.1 Vessel density quantification. (a) Illustration of vessel density measurement in defined sub-fields; (b) Box-and-whisker plot of vessel density measurement and correspongding p value.

 
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