June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Assessment of the Potential for Introducing Bilateral Injury Using the TITON Apparatus
Author Affiliations & Notes
  • Brooke Ivie Asemota
    Saint Louis University School of Medicine, Saint Louis, MO
    Biomedical Engineering, University of Texas at San Antonio, San Antonio, TX
  • Luis Rodriguez
    Biomedical Engineering, University of Texas at San Antonio, San Antonio, TX
  • Matthew Aaron Reilly
    Biomedical Engineering, University of Texas at San Antonio, San Antonio, TX
  • Footnotes
    Commercial Relationships Brooke Asemota, None; Luis Rodriguez, None; Matthew Reilly, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 6022. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Brooke Ivie Asemota, Luis Rodriguez, Matthew Aaron Reilly; Assessment of the Potential for Introducing Bilateral Injury Using the TITON Apparatus. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):6022.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

Traumatic optic neuropathy (TON) occurs in up to 5% of all head traumas resulting in severe visual deficit or blindness. In this study bilateral torsional indirect TON was induced in a physiologically relevant rat model, which may be used for development of novel therapeutics.

 
Methods
 

A torsional indirect TON insult was applied to both eyes using the TITON apparatus (Asemota, B. I. et al., ARVO 2014 E-abstract 55:6194). Flash visual evoked potentials (fVEPs) were used before and after injury to assess the performance of the retinal ganglia and optic nerves. The amplitude and velocity of the insults were varied to modulate the bilateral symmetry of TON. H0: The changes in fVEPs were different between eyes in the same animal.

 
Results
 

The TITON apparatus was capable of inducing bilateral TON. Modulation of the parametric values of the insults resulted in the presentation of bilaterally symmetrical TON. P-values for H0: P1-N1 amplitude = 0.668625, P1-N1 latency = 0.516895, P2-N2 amplitude = 0.964479, P2-N2 latency = 0.725215.

 
Conclusions
 

The TITON apparatus is capable of inducing bilaterally symmetrical TON. Further modulation of the parametric values resulted in bilaterally non-symmetrical TON. These findings suggest that the model can be used in the study and development of neuroprotectives for the optic nerve and retinal ganglion cells. Controlled production of bilaterally non-symmetric TON may prove highly valuable for future research involving the replication of medically observed TON for more specialized treatment purposes.  

 
Figure 1: Data acquisition from an fVEP waveform showing P1, N1, P2, N2. Latency and Amplitude were determined for both P1-N1 and P2-N2.
 
Figure 1: Data acquisition from an fVEP waveform showing P1, N1, P2, N2. Latency and Amplitude were determined for both P1-N1 and P2-N2.

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×