June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Quantitative analysis of microvascular network of the lid wiper area in contact lens wearers
Author Affiliations & Notes
  • Jianhua Wang
    Ophthalmology, Bascom Palmer Eye Inst Lib, Miami, FL
  • Hong Jiang
    Ophthalmology, Bascom Palmer Eye Inst Lib, Miami, FL
  • Xinjie Mao
    Ophthalmology, Bascom Palmer Eye Inst Lib, Miami, FL
    Ophthalmology and Optometry, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Wentao Yan
    Ophthalmology and Optometry, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Footnotes
    Commercial Relationships Jianhua Wang, NIH (F); Hong Jiang, NIH (F); Xinjie Mao, None; Wentao Yan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 6068. doi:
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      Jianhua Wang, Hong Jiang, Xinjie Mao, Wentao Yan; Quantitative analysis of microvascular network of the lid wiper area in contact lens wearers. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):6068.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To demonstrate the feasibility of quantitative analysis of microvascular network of the lid wiper area of the human eye imaged with our functional slit-lamp biomicroscopy (FSLB).

 
Methods
 

FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the conjunctiva to take images of the lid wiper area, which were compared to the images of the tarsus. Nine human subjects including 3 habitual contact lens wearers were imaged twice at one visit. Only the left eye was imaged. Custom software for automated segmenting the microvascular network has been developed and described in our previous publication (Jiang et al. Microvascular Research, 2014;92:62-71). The same software was used to segment the microvascular network of the lid wiper and tarsus. We used the fractal analysis toolbox from Benoit™ (TruSoft Benoit Pro 2.0, TruSoft Inc., St. Petersburg, FL) to analyze the fractal dimension of the microvascular network. Multifractal analysis (D0) was performed to quantify the fractality (density and complexity) of the vascular network of the lid wiper area (Fig. 1) and the tarsal conjunctiva (Fig. 2). The coefficient of repeatability (CoR) of the tests was analyzed.

 
Results
 

D0 of the lid wiper area was 1.556 ± 0.209 (mean ± SD) for the first session and 1.572 ± 0.213 for the second session. The CoR of these measurements was 7.7%. D0 was 1.358 (SD: 0.213) in contact lens wearers and 1.667 (SD: 0.116) in healthy subjects (P = 0.057). D0 of the tarsus was 1.706 ± 0.053 for the first time and 1.709 ± 0.053 for the second time. The CoR of these measurements was 3.5%. D0 was 1.703 (SD: 0.005) in contact lens wearers and 1.719 (SD: 0.064) in healthy subjects (P = 0.246). The difference of the microvascular network was significant (P = 0.046) between the lid wiper and tarsus.

 
Conclusions
 

This is the first time to demonstrate the feasibility of quantitatively analyzing the microvascular network of the lid wiper area and the tarsal area using FSLB. Further studies will need to be done on a large sample of subjects and patients with diseases and conditions. The approach could be applied to study dry eye, contact lens wear and other ocular surface diseases involving the damage and inflammation of the lid wiper and tarsal conjunctiva.  

 

 
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