June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Addition of Laminar Beams Into the Lamina Cribrosa Structure in Experimental Glaucoma
Author Affiliations & Notes
  • Massimo Antonio Fazio
    Ophthalmology, University of Alabama at Birmingham, Birmingham, AL
  • Vincent Libertiaux
    Ophthalmology, University of Alabama at Birmingham, Birmingham, AL
  • Hongli Yang
    Optic Nerve Head Research Laboratory, Devers Eye Institue, Portland, OR
  • Howard Lockwood
    Optic Nerve Head Research Laboratory, Devers Eye Institue, Portland, OR
  • Juan Reynaud
    Optic Nerve Head Research Laboratory, Devers Eye Institue, Portland, OR
  • Claude F Burgoyne
    Optic Nerve Head Research Laboratory, Devers Eye Institue, Portland, OR
  • J Crawford C Downs
    Ophthalmology, University of Alabama at Birmingham, Birmingham, AL
  • Footnotes
    Commercial Relationships Massimo Fazio, None; Vincent Libertiaux, None; Hongli Yang, None; Howard Lockwood, None; Juan Reynaud, None; Claude Burgoyne, Heidelberg Engineering (F), Heidelberg Engineering (R); J Crawford Downs, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 6154. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Massimo Antonio Fazio, Vincent Libertiaux, Hongli Yang, Howard Lockwood, Juan Reynaud, Claude F Burgoyne, J Crawford C Downs; Addition of Laminar Beams Into the Lamina Cribrosa Structure in Experimental Glaucoma. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):6154.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To quantify the regional differences in the density, orientation, degree of anisotropy, thickness, and number of beams through the thickness of the lamina cribrosa in nonhuman primate (NHP) eyes with EG compared to their normal contralateral controls.

 
Methods
 

Four bilateral normal and 9 NHPs with induced unilateral glaucoma were sacrificed at the onset of early EG (10-30% axon loss).2,3 ONHs underwent 3D reconstruction1,3 The images are then aligned and stacked into a digital 3D ONH reconstructions. The lamina cribrosa (LC) microarchitecture was delineated, segmented and sampled on a discrete grid to compute the LC connective tissue volume (CTV), LC connective tissue volume fraction (CTVF), the degree of anisotropy, the predominant laminar beam orientation, and the number of beams through the thickness of the LC within each sampling volume, which were regionalized into peripheral and central LC regions of equal area. Linear mixed effect statistical models were used to analyze the effects of treatment on the outcome variables.

 
Results
 

The number of laminar beams through the thickness of the LC was significantly higher in the EG eyes compared to the normal controls (16%, p<0.001), and this effect was larger in the peripheral laminar region (Figure). CTV was also significantly increased by 17% in the EG eyes (p=0.005), as was LC thickness (28%, p<0.001). Anisotropy was significantly lower in the EG eyes (-9%, p<0.05). CTVF was not significantly different by treatment.

 
Conclusions
 

Results suggest that the number of beams through the thickness of the LC increases in early EG. These data, along with changes in LC anisotropy, CTVF, and thickness indicate widespread LC connective tissue remodeling and restructuring early in glaucoma, which likely changes its biomechanical response to IOP.<br /> <br /> 1Yang H, et al. IOVS 2009;50:224-234<br /> 2He L, et al. IOVS 2014;55:574-586<br /> 3Yang H, et al. IOVS 2011;52:345-363  

 
Box-whisker plots showing the treatment effect of experimental glaucoma on the number of beams through the thickness of the LC and the degree of anisotropy in the LC for central and peripheral regions in both early EG (GL) and contralateral control (Norm) eyes.<br /> Box-and-whisker plot summarizing: Upper and Lower Fence; Q1, Median, and Q3; Mean ±95% CI.
 
Box-whisker plots showing the treatment effect of experimental glaucoma on the number of beams through the thickness of the LC and the degree of anisotropy in the LC for central and peripheral regions in both early EG (GL) and contralateral control (Norm) eyes.<br /> Box-and-whisker plot summarizing: Upper and Lower Fence; Q1, Median, and Q3; Mean ±95% CI.

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×