June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Racial Differences in the Extracellular Matrix of the Lamina Cribrosa and Peripapillary Sclera: Implications to Normal-Tension Glaucoma
Author Affiliations & Notes
  • Hae-Young Lopilly Park
    Department of Ophthalmology, Catholic University of Korea, Seoul, Korea (the Republic of)
  • Chan Kee Park
    Department of Ophthalmology, Catholic University of Korea, Seoul, Korea (the Republic of)
  • Jie Hyun Kim
    Department of Ophthalmology, Catholic University of Korea, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships Hae-Young Park, None; Chan Kee Park, None; Jie Hyun Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 6159. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Hae-Young Lopilly Park, Chan Kee Park, Jie Hyun Kim; Racial Differences in the Extracellular Matrix of the Lamina Cribrosa and Peripapillary Sclera: Implications to Normal-Tension Glaucoma. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):6159.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

The exact pathogenesis of normal-tension glaucoma is unknown, and evidence shows that the properties of the lamina cribrosa and sclera may play a role. In this study, we investigated the major extracellular matrix (ECM) components of the lamina cribrosa and sclera in human donor eyes to determine the differences between Korean and Caucasian eyes.

 
Methods
 

Quantitative RT-PCR was performed with the total RNA extracted from the retina, choroid, sclera, peripapillary sclera (2 mm surrounding the lamina cribrosa), lamina cribrosa, and the optic nerve to quantitatively evaluate the ECM protein composition in ocular tissues. The collagen and elastic fiber systems were analyzed qualitatively and quantitatively in cross-sections of the lamina cribrosa. Collagen fibers stained with picrosirius red and elastic fibers stained with resorcin-fuchsin stain. Indirect immunofluorescence single- or double-labeling was performed on cryosections of posterior segment tissues using primary antibodies against lysly oxidase, lysyl oxidase-like 1, lysly oxidase-like 2, elastin, and fibrillin-1. Ultrathin sections (70-90 nm) were taken from representative areas, placed on copper mesh grids (Electron Microscopy Sciences), and examined using an electron microscope. The stress-stain relationship was measured using the Biomaterial Universal testing machine.

 
Results
 

Collagen and elastin were significantly more abundant in Korean eyes as measured by quantitative polymerase chain reaction and immunohistochemical staining. The morphology of the collagen and elastic fibers as visualized by special stains and electron microscopy differed by race. Collagen fibers had a greater preferred directionality and smaller fibril diameter in Korean eyes. Elastic fibers in Korean eyes formed round aggregates around the laminar beams and shorter, ill-defined or coiled-like fibril structures. The mechanical properties of the ONH tissues produced greater strain in Korean eyes.

 
Conclusions
 

Therefore, the lamina cribrosa and peripapillary sclera are more easily deformed by similar pressures in Korean eyes compared to Caucasian eyes. Racial differences in the ECM composition and microscopic architecture may contribute to the greater deformation of the ONH tissues under the normal IOP range and the prevalence of NTG in Asian eyes.  

 

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×