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Narges Fazili, Sahar Balagholi, Mozhgan Rezaeikanavi, Somayeh Asadi, Seyed Bagher Hosseini; Adherence Capability of Cultivated Retinoblastoma Cells. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):66.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate adherence capability of cultivated retinoblastoma (RB) tumorspheres with prolonged cultivation.
RB cells from two Iranian patients were cultivated in DMEM supplemented with 15% FBS for four weeks in each passage. Fresh medium was added on a weekly basis and immunocytochemistry for synaptophysin was performed. All the experiments were done in duplicate. Cell attachment capability was studied during three consecutive passages.
A biphasic population of cultivated cells was observed during the first week in each passage; composed of RB tumorspheres and a single-cell suspension overlying a layer of fibroblastic cells that had adhered to the bottom of flask. Early adherence of RB tumorspheres to the bottom of flask, while surrounded by fibroblasts, was observed in the second week and increased by the fourth week (figure).
Compared to previous data, this study demonstrated the adherence capability of RB tumorspheres to the underlying surface with prolonged cultivation; which after 4 weeks seems to be independent of the fibroblasts.
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