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Long Shi, Xinyi Wu; JNK Signaling Mediates CTGF Expression and Scar Formation in Corneal Wound Healing. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):739.
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To investigate the signaling pathway mediates the expression of Connective Tissue Growth Factor (CTGF) and corneal scarring in corneal wound healing
We investigated whether TGF-β1 could induced MAPK pathways hosphorylation in Telomerase-immortalized human cornea stroma fibroblasts (THSF), and determined the effect of the MAPK pathways in TGF-β1 induced CTGF, fibronectin and collagen I expression. Then, the penetrating corneal wound model was created in vivo and the effect of JNK on CTGF expression and corneal scarring in corneal wound healing was identified.
TGF-β1 activated MAPK pathways in THSF cells. JNK inhibitor significantly inhibited CTGF, fibronectin and collagen I expression induced by TGF-b1 in THSF. In corneal wound healing, the JNK inhibitor significantly inhibited CTGF expression, markedly improved the architecture of corneal stroma and reduced corneal scar formation, but did not have a measurable impact on corneal wound healing in vivo.
JNK mediates the expression of CTGF and corneal scarring in corneal wound healing, and might be considered as specific targets of drug therapy for corneal scarring.
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