June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Lamina Cribrosa Position in the Non-Human Primate (NHP) Optic Nerve Transection (ONT) Model of a Non-Glaucomatous Optic Neuropathy
Author Affiliations & Notes
  • Eliesa Ing
    Glaucoma, Devers Eye Institute, Portland, OR
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Kevin Ivers
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Hongli Yang
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Stuart Keith Gardiner
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Juan Reynaud
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Grant Cull
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Lin Wang
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Claude F Burgoyne
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Portland, OR
  • Footnotes
    Commercial Relationships Eliesa Ing, None; Kevin Ivers, None; Hongli Yang, None; Stuart Gardiner, None; Juan Reynaud, None; Grant Cull, None; Lin Wang, None; Claude Burgoyne, Heidelberg Engineering (C), Heidelberg Engineering (F), Heidelberg Engineering (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 999. doi:
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      Eliesa Ing, Kevin Ivers, Hongli Yang, Stuart Keith Gardiner, Juan Reynaud, Grant Cull, Lin Wang, Claude F Burgoyne; Lamina Cribrosa Position in the Non-Human Primate (NHP) Optic Nerve Transection (ONT) Model of a Non-Glaucomatous Optic Neuropathy. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):999.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

In 2 recent NHP studies, Spectral Domain Optical Coherence Tomography (SDOCT) detected posterior lamina cribrosa deformation prior to RNFLT change following chronic experimental IOP elevation1 but failed to demonstrate lamina cribrosa deformation 2 years following chronic experimental cerebrospinal fluid (CSF) pressure lowering.2 The purpose of this study is to use SDOCT to longitudinally characterize lamina cribrosa position in the NHP ONT model.

 
Methods
 

Both eyes of 5 adult NHPs underwent SDOCT imaging 6 times at baseline, then biweekly following unilateral ONT surgery for 1.5 to 2 months, when animals were sacrificed as per their primary study. The internal limiting membrane and Bruch’s Membrane Opening (BMO) were delineated in 48 ONH B-scans from each pre and post-ONT imaging session. Global ONH minimum rim width (MRW) and RNFL thickness (RNFLT - 12° circular scans) were calculated and compared to their baseline 95% confidence interval (95% CI) to determine change. The anterior laminar cribrosa surface (ALCS) was delineated within the baseline and pre-sacrifice SDOCT data sets. Pre-sacrifice ALCS depth relative to a BMO reference plane (ALCSD) was then compared to its baseline 95% CI to determine ALCSD change.

 
Results
 

SDOCT data for 3 ONT animals (M1, M2 & M3) have been analyzed. Pre and post-ONT global MRW, RNFLT and ALCSD data (pre-sacrifice only) for both eyes of each animal are shown in Fig 1. Representative ONT eye baseline and pre-sacrifice SDOCT B-scans from each animal are shown in Fig 2. Pre-sacrifice percent change in MRW, RNFLT, and ALSCD was -31.3%, -45.0%, +3.1% for M1; -25.2%, -41.4%, and +7.5% for M2; and -26.3%, -39.4%, and +28.8% for M3, respectively. ALCSD stayed the same in M1&M2, and moved anteriorly in M3. Data for all 5 animals will be presented at the meeting.

 
Conclusions
 

In a preliminary analysis of 3 ONT animals, while SDOCT MRW and RNFLT demonstrated profound change within the ONT eyes, ALCSD was unchanged or anteriorly displaced from baseline at 51 (M1), 49 (M2), and 50 (M3) days post transection. Assuming our findings are confirmed in all 5 ONT animals, we propose that posterior laminar deformation should be included in the definition of a glaucomatous vs non-glaucomatous optic neuropathy in the NHP eye.<br /> <br /> 1. He, et al. IOVS 2014;55:574-586<br /> 2. Yang, et al. IOVS 2014;55:3067-3073  

 

 
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